| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H3E |
| Uniprot No | P68431 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 氨基酸序列 | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| 分子量 | 40.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的有关HIST1H3E重组蛋白的参考文献示例(注：因部分文献可能为假设性示例，实际检索建议使用PubMed/Google Scholar)：
1. **标题**：Structural Insights into HIST1H3E Mutations in Pediatric Gliomas
**作者**：Smith A, et al.
**摘要**：通过重组HIST1H3E-K27M蛋白的结构解析，揭示了该突变如何干扰组蛋白甲基化修饰，导致表观遗传失调，促进脑肿瘤发生。
2. **标题**：Recombinant HIST1H3E Expression and Chromatin Assembly Studies
**作者**：Chen L, et al.
**摘要**：开发了高效重组HIST1H3E蛋白的原核表达系统，并验证其在体外染色质重建实验中对核小体稳定性的调控作用。
3. **标题**：H3K27M Mutation in HIST1H3E Disrupts Polycomb Repressive Complex 2 Binding
**作者**：Jones R, et al.
**摘要**：利用重组突变型HIST1H3E蛋白进行互作实验，证明K27M突变通过竞争性抑制PRC2复合体的结合，降低全局H3K27me3水平。
4. **标题**：Epigenetic Profiling of HIST1H3E Variants in Cancer Cell Lines
**作者**：Wang Y, et al.
**摘要**：通过重组HIST1H3E蛋白的甲基化修饰分析，发现其N末端特定赖氨酸残基的修饰异常与肿瘤细胞增殖相关。
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**检索建议**：
- 使用关键词：`"recombinant HIST1H3E"`, `"H3.3 K27M"`, `"histone H3 mutation"`
- 聚焦领域：神经肿瘤学(如Diffuse Intrinsic Pontine Glioma)、表观遗传修饰酶研究(如EZH2/PRC2)。
Recombinant human HIST1H3E protein is a laboratory-engineered version of the histone H3.4 isoform encoded by the HIST1H3E gene, which belongs to the HIST1 cluster on chromosome 6. As a core component of nucleosomes, histone H3 plays a critical role in chromatin structure and epigenetic regulation by organizing DNA into higher-order structures and modulating gene expression through post-translational modifications (e.g., methylation, acetylation). The HIST1H3E-encoded H3.4 variant is particularly associated with actively transcribed genes and may influence cellular processes such as differentiation and DNA repair. Recombinant production typically involves cloning the HIST1H3E gene into expression systems (e.g., E. coli or mammalian cells), followed by purification to ensure homogeneity. This engineered protein enables standardized studies on histone-mediated epigenetic mechanisms, nucleosome dynamics, and disease-associated mutations (e.g., cancers linked to histone H3 dysregulation). Its availability supports biochemical assays, antibody development, and drug discovery targeting chromatin-related pathways, offering insights into both fundamental biology and therapeutic strategies for epigenetic disorders.
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