| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H4C |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-103aa |
| 氨基酸序列 | MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG |
| 分子量 | 11.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST1H4C蛋白的3篇示例参考文献(注：部分内容为虚构，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Expression and Purification of Recombinant Human HIST1H4C for Chromatin Assembly Studies*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了通过大肠杆菌系统表达重组人HIST1H4C蛋白的高效方法，并优化了纯化流程。通过体外染色质重建实验，验证了该重组蛋白与组蛋白H3的结合能力，为研究核小体动力学提供了工具。
2. **文献名称**: *Functional Analysis of HIST1H4C Mutations in Epigenetic Regulation*
**作者**: Kumar S, Patel R.
**摘要**: 文章利用重组HIST1H4C蛋白(携带K8R和G13D突变)探究组蛋白修饰对基因沉默的影响。实验表明，突变体显著降低H4K16乙酰化水平，并影响染色质压缩过程。
3. **文献名称**: *HIST1H4C Interaction with Histone Chaperone ASF1B Revealed by Recombinant Protein Assays*
**作者**: Müller J, et al.
**摘要**: 作者通过表面等离子共振技术(SPR)验证了重组HIST1H4C与伴侣蛋白ASF1B的相互作用，揭示了ASF1B在H4-H3四聚体组装中的关键作用。
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**提示**：如需真实文献，建议在PubMed或Web of Science中检索关键词(如“recombinant HIST1H4C”或“histone H4C expression”)，并结合实际研究需求筛选。
The human HIST1H4C protein is a member of the histone H4 family, essential for chromatin structure and epigenetic regulation. Histones are core components of nucleosomes, around which DNA wraps to form chromatin. The H4 family comprises multiple isoforms encoded by genes clustered in the HIST1 locus, with HIST1H4C being one of several H4 variants. This 102-amino-acid protein contains a conserved globular domain and a flexible N-terminal tail, which undergoes post-translational modifications (e.g., acetylation, methylation) that regulate chromatin compaction, gene expression, and DNA repair.
Recombinant HIST1H4C is produced via genetic engineering, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems to ensure proper folding and post-translational modification capabilities. Its recombinant form enables studies on nucleosome assembly, histone-modifying enzymes, and epigenetic mechanisms in diseases such as cancer, where altered H4 expression or modifications are linked to transcriptional dysregulation. Researchers also use it to investigate chromatin remodeling complexes and develop therapeutics targeting epigenetic pathways.
As a core histone, HIST1H4C’s recombinant version retains structural and functional properties of native H4. often tagged (e.g., His-tag) for purification and detection. Its study advances understanding of genome organization, cellular differentiation, and disease-associated chromatin abnormalities.
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