| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H3C |
| Uniprot No | Q71DI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 氨基酸序列 | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| 分子量 | 41.91 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人HIST2H3C蛋白的虚构参考文献示例(注：由于HIST2H3C相关研究较为细分，实际文献需通过PubMed/Google Scholar验证)：
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1. **文献名称**: "Expression and purification of recombinant human HIST2H3C in E. coli for chromatin remodeling studies"
**作者**: Zhang, L., et al.
**摘要**: 本研究建立了一种利用大肠杆菌系统高效表达重组人HIST2H3C蛋白的方法，通过His标签亲和层析纯化，验证其与DNA结合能力及在体外核小体重组实验中的功能。
2. **文献名称**: "Post-translational modifications of HIST2H3C in human cancer epigenetics"
**作者**: Gupta, R., & Patel, S.
**摘要**: 分析了重组HIST2H3C蛋白在体外的甲基化/乙酰化修饰模式，揭示其K36位点异常甲基化与乳腺癌表观遗传失调的相关性。
3. **文献名称**: "Structural insights into HIST2H3C variants using recombinant protein crystallography"
**作者**: Müller, F., et al.
**摘要**: 通过表达重组HIST2H3C蛋白并进行X射线晶体学分析，解析了其N端结构域与组蛋白伴侣ASF1的互作机制，为染色质组装提供结构基础。
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*注：以上为模拟文献，具体研究请检索真实数据库(如PMID或DOI编码)。如需真实文献方向建议，可关注：组蛋白重组表达技术、H3亚型功能分化、表观遗传修饰相关研究。*
**Background of Recombinant Human HIST2H3C Protein**
The HIST2H3C gene encodes histone H3.2. a core component of nucleosomes that organizes eukaryotic DNA into chromatin. As part of the H3 histone family, H3.2 is essential for DNA packaging, chromosomal stability, and epigenetic regulation. Unlike replication-dependent histone variants (e.g., H3.1) or replacement variants (e.g., H3.3), H3.2 is primarily expressed during the S phase of the cell cycle and shares high sequence homology with H3.1. It contains a conserved globular domain and a flexible N-terminal tail, which undergoes post-translational modifications (e.g., acetylation, methylation) to modulate gene expression, DNA repair, and cell differentiation.
Recombinant human HIST2H3C protein is produced *in vitro* using expression systems like *E. coli* or yeast, often fused with tags (e.g., His-tag) for purification. Its production enables studies on histone-DNA interactions, chromatin remodeling, and enzyme kinetics (e.g., histone acetyltransferases or methyltransferases). Researchers use it in epigenetics, cancer biology (aberrant H3 modifications link to malignancies), and drug discovery (targeting histone-modifying enzymes). As a standardized reagent, it also aids in developing diagnostic tools or exploring therapies for diseases linked to epigenetic dysregulation.
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