| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRPM |
| Uniprot No | P52272 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-730aa |
| 氨基酸序列 | MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA |
| 分子量 | 103.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HNRPM(HNRNPM)蛋白的参考文献示例(文献为虚构示例，仅作格式参考)：
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1. **文献名称**：*Recombinant HNRNPM regulates alternative splicing by binding to intronic RNA motifs*
**作者**：Han, S.P. et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备重组HNRNPM蛋白，并证明其通过特异性结合内含子区域的RNA基序调控基因的选择性剪接，为剪接机制提供了新见解。
2. **文献名称**：*Structural analysis of the RNA-binding domains in recombinant HNRNPM*
**作者**：Huang, L. et al.
**摘要**：利用重组表达的人源HNRNPM蛋白进行X射线晶体学分析，揭示了其RNA结合域的三维结构，阐明了其与靶标RNA相互作用的分子机制。
3. **文献名称**：*HNRNPM promotes HIV-1 replication via interaction with viral RNA*
**作者**：Zhou, Y. et al.
**摘要**：通过重组HNRNPM蛋白的体外结合实验，证实其直接结合HIV-1 RNA的特定区域，增强病毒转录效率，为抗病毒治疗提供潜在靶点。
4. **文献名称**：*Overexpression of recombinant HNRNPM correlates with tumor progression in breast cancer*
**作者**：Pino, M.S. et al.
**摘要**：在乳腺癌细胞中过表达重组HNRNPM蛋白，发现其通过稳定促癌基因mRNA促进细胞增殖，提示其作为癌症生物标志物的潜力。
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**注**：上述文献及摘要均为示例，实际研究中请根据具体课题检索权威数据库(如PubMed、Web of Science)获取真实参考文献。
**Background of Recombinant Human HNRNPM Protein**
Heterogeneous nuclear ribonucleoprotein M (HNRNPM) is a member of the hnRNP family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, transport, stability, and translation. HNRNPM specifically binds to RNA through its RNA recognition motifs (RRMs) and glycine-rich domains, enabling interactions with diverse RNA targets. It regulates alternative splicing by modulating spliceosome assembly and interacts with other proteins to influence mRNA export and localization. HNRNPM is also implicated in DNA repair and transcriptional regulation, underscoring its multifunctionality in gene expression.
Dysregulation of HNRNPM is linked to diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. Its overexpression in certain cancers correlates with tumor progression, metastasis, and therapy resistance, making it a potential therapeutic target.
Recombinant human HNRNPM protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. This engineered protein retains native biological activity, enabling *in vitro* studies on its molecular mechanisms, RNA-protein interactions, and role in disease pathways. It is widely used in structural analyses, drug screening, and functional assays to explore HNRNPM’s involvement in cellular processes. As a research tool, recombinant HNRNPM aids in deciphering its regulatory networks and validating diagnostic or therapeutic strategies targeting RNA-processing pathways.
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