| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRPDL |
| Uniprot No | O14979 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420aa |
| 氨基酸序列 | MEVPPRLSHVPPPLFPSAPATLASRSLSHWRPRPPRQLAPLLPSLAPSSARQGARRAQRHVTAQQPSRLAGGAAIKGGRRRRPDLFRRHFKSSSIQRSAAAAAATRTARQHPPADSSVTMEDMNEYSNIEEFAEGSKINASKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKEHKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFITYTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQKGGRGAAAGGRGGTRGRGRGQGQNWNQGFNNYYDQGYGNYNSAYGGDQNYSGYGGYDYTGYNYGNYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY |
| 分子量 | 72.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HNRPDL(hnRNPDL)蛋白的3篇代表性文献的模拟示例(基于研究领域常见方向，非真实文献)：
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1. **文献名称**：*hnRNPDL regulates RNA splicing and stability in neural development*
**作者**：Smith A, et al.
**摘要**：本研究通过重组表达人hnRNPDL蛋白，分析了其在神经元分化中的功能，发现该蛋白通过结合特定RNA序列调控剪接和稳定性，影响轴突导向相关基因的表达。
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2. **文献名称**：*Structural basis of hnRNPDL’s RNA-binding specificity revealed by X-ray crystallography*
**作者**：Zhang L, et al.
**摘要**：通过重组表达并纯化人hnRNPDL的RNA结合结构域，结合X射线晶体学解析其结构，揭示了其识别RNA基序的分子机制，为理解RNA代谢疾病提供结构依据。
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3. **文献名称**：*Mutations in hnRNPDL disrupt phase separation in limb-girdle muscular dystrophy*
**作者**：Chen X, et al.
**摘要**：研究发现与肢带型肌营养不良相关的hnRNPDL突变体(通过重组蛋白表达)破坏液-液相分离能力，导致异常RNA聚集，影响肌肉细胞中应激颗粒的形成。
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**备注**：
以上文献示例为模拟生成，反映hnRNPDL研究的典型方向(RNA代谢、结构生物学、疾病关联)。实际检索时可通过 **PubMed** 或 **Google Scholar** 输入关键词(如“recombinant hnRNPDL protein”或“HNRPDL function”)获取真实文献。若需具体文章，请提供更详细的研究方向。
**Background of Recombinant Human HNRPDL Protein**
Heterogeneous nuclear ribonucleoprotein D-like (HNRPDL), also known as hnRNPDL or JKTBP, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. These proteins play critical roles in RNA metabolism, including splicing, transport, stability, and translation. HNRPDL contains RNA recognition motifs (RRMs) that enable binding to nucleic acids, particularly GU-rich regions in pre-mRNA and mRNA, influencing gene expression at transcriptional and post-transcriptional levels.
HNRPDL functions as a regulatory switch, modulating alternative splicing and mRNA stability in response to cellular signals. It is ubiquitously expressed, with high levels in muscles and the nervous system. Mutations in the *HNRPDL* gene are linked to limb-girdle muscular dystrophy and other neuromuscular disorders, underscoring its importance in muscle integrity and cellular homeostasis.
Recombinant human HNRPDL protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. It serves as a vital tool for studying RNA-protein interactions, dissecting molecular mechanisms in RNA processing, and exploring therapeutic strategies for related diseases. Its application extends to *in vitro* assays, structural studies, and disease modeling, aiding advancements in molecular biology and medicine.
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