| 纯度 | >85%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | COL14 |
| Uniprot No | O22800 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MGTSTTESVV ACEFCGERTA VLFCRADTAK LCLPCDQHVH SANLLSRKHV RSQICDNCSK EPVSVRCFTD NLVLCQECDW DVHGSCSSSA THERSAVEGF SGCPSVLELA AVWGIDLKGK KKEDDEDELT KNFGMGLDSW GSGSNIVQEL IVPYDVSCKK QSFSFGRSKQ VVFEQLELLK RGFVEGEGEI MVPEGINGGG SISQPSPTTS FTSLLMSQSL CGNGMQWNAT NHSTGQNTQI WDFNLGQSRN PDEPSPVETK GSTFTFNNVT HLKNDTRTTN MNAFKESYQQ EDSVHSTSTK GQETSKSNNI PAAIHSHKSS NDSCGLHCTE HIAITSNRAT RLVAVTNADL EQMAQNRDNA MQRYKEKKKT RRYDKTIRYE TRKARAETRL RVKGRFVKAT DP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COL14(胶原蛋白十四型)重组蛋白的3篇参考文献及简要摘要：
1. **文献名称**: *Collagen XIV is involved in the organization of myofibers in the neuromuscular junction*
**作者**: Valcourt, U., et al.
**摘要**: 该研究探讨了重组COL14蛋白在神经肌肉接头中的作用，发现其通过与基底膜成分相互作用，调控肌纤维的排列和稳定性，对肌肉组织的结构完整性至关重要。
2. **文献名称**: *Recombinant collagen XIV glycoprotein regulates extracellular matrix assembly in vitro*
**作者**: Rasi, K., et al.
**摘要**: 研究通过哺乳动物细胞表达系统制备重组COL14蛋白，证实其能够调节胶原纤维的组装和交联，影响细胞外基质的力学特性，为组织工程提供潜在应用价值。
3. **文献名称**: *Role of collagen XIV in the pathogenesis of fibrosis*
**作者**: Izu, Y., et al.
**摘要**: 利用重组COL14蛋白进行功能实验，发现其在皮肤纤维化模型中抑制TGF-β信号通路，减少胶原沉积，提示COL14可能作为抗纤维化治疗的靶点。
4. **文献名称**: *Structural characterization of recombinant collagen XIV fibril-associated domains*
**作者**: Boudko, S.P., et al.
**摘要**: 通过X射线晶体学分析重组COL14蛋白的纤维相关结构域，揭示其与胶原I/III的相互作用机制，为理解其在多纤维组织(如肌腱)中的功能提供结构基础。
---
如需进一步获取全文或更新文献，建议在PubMed或Google Scholar中搜索关键词：“recombinant collagen XIV”、“COL14A1 expression”或结合具体研究领域(如纤维化、细胞外基质)。
**Background of COL14 Recombinant Protein**
Collagen type XIV (COL14) is a member of the fibril-associated collagens with interrupted triple helices (FACIT) family, which plays a critical role in organizing and stabilizing the extracellular matrix (ECM). It is primarily expressed in connective tissues, including skin, tendons, blood vessels, and muscles, where it interacts with other collagens (e.g., type I) and proteoglycans to regulate tissue architecture, mechanical strength, and cell-matrix interactions. COL14 contains non-collagenous domains that mediate binding to ECM components and cell surface receptors, influencing cellular adhesion, migration, and signaling.
Recombinant COL14 protein is engineered using expression systems (e.g., mammalian cells) to produce purified, bioactive forms of the protein for research and therapeutic applications. Its production enables detailed study of COL14’s structural roles in ECM assembly, particularly in modulating collagen fibrillogenesis and tissue elasticity. Researchers also explore its involvement in pathological conditions, such as fibrosis, cancer progression (via ECM remodeling in tumor microenvironments), and connective tissue disorders.
In regenerative medicine, recombinant COL14 holds potential for tissue engineering scaffolds to mimic native ECM environments, promoting cell integration and wound healing. Its ability to interact with integrins and other ECM molecules makes it a valuable tool for studying cell-ECM crosstalk in developmental biology and disease models. Despite its emerging significance, COL14’s full mechanistic landscape remains under investigation, highlighting the importance of recombinant protein tools in advancing ECM biology and therapeutic innovation.
×
