| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS1BP3 |
| Uniprot No | Q53T59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MQSPAVLVTSRRLQNAHTGLDLTVPQHQEVRGKMMSGHVEYQILVVTRLAAFKSAKHRPEDVVQFLVSKKYSEIEEFYQKLSSRYAAASLPPLPRKVLFVGESDIRERRAVFNEILRCVSKDAELAGSPELLEFLGTRSPGAAGLTSRDSSVLDGTDSQTGNDEEAFDFFEEQDQVAEEGPPVQSLKGEDAEESLEEEEALDPLGIMRLVSCC |
| 分子量 | 49.17 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HS1BP3蛋白的3篇参考文献概览：
1. **文献名称**：*HS1BP3 regulates cell proliferation and survival through modulating phosphoinositide turnover*
**作者**：Chen L., et al.
**摘要**：研究揭示了重组人HS1BP3蛋白通过与磷脂酰肌醇信号通路相互作用，调控细胞增殖和凋亡的分子机制，实验表明其异常表达可能影响癌症进展。
2. **文献名称**：*Structural characterization and functional analysis of recombinant human HS1BP3 in cellular vesicle trafficking*
**作者**：Tanaka K., et al.
**摘要**：该研究利用重组HS1BP3蛋白进行结构解析和功能实验，证明其参与细胞内囊泡运输过程，并揭示其BAR结构域在膜变形中的关键作用。
3. **文献名称**：*HS1BP3 as a novel binding partner of 5-lipoxygenase: Implications for inflammatory responses*
**作者**：Müller S., et al.
**摘要**：文章通过重组蛋白互作实验发现HS1BP3与5-脂氧合酶(5-LO)直接结合，调控炎症反应中白三烯的合成，提示其在炎症性疾病中的潜在治疗靶点。
(注：以上文献信息为示例，实际引用需依据真实发表论文调整。)
HS1BP3 (Hematopoietic lineage cell-specific protein 1-binding protein 3), also known as HS1-binding protein 3 or HAX1-interacting protein, is a regulatory protein involved in diverse cellular processes. It interacts with HS1 (or Lck-associated substrate homolog), a cytoskeletal adaptor protein critical for immune cell signaling, migration, and apoptosis. Structurally, HS1BP3 contains conserved domains, including a pleckstrin homology (PH) domain, suggesting roles in membrane localization or protein-protein interactions.
Studies indicate HS1BP3 modulates HS1-mediated signaling pathways in hematopoietic cells, particularly influencing B-cell receptor signaling and lymphocyte activation. It also associates with HAX1. an anti-apoptotic protein, implicating its involvement in mitochondrial homeostasis and cell survival. Dysregulation of HS1BP3 has been linked to pathologies such as leukemia, autoimmune disorders, and neurodegenerative diseases, though mechanistic details remain under investigation.
Recombinant human HS1BP3 protein is typically produced in bacterial or mammalian expression systems, enabling functional studies. Its purified form facilitates in vitro analyses, including binding assays, structural characterization, and identification of interaction partners. Researchers utilize it to explore HS1BP3's role in immune regulation, apoptosis, and intracellular trafficking, offering potential insights into therapeutic strategies targeting hematologic malignancies or immune dysregulation. Its application extends to antibody development and screening platforms for drug discovery. Further research is needed to fully elucidate its molecular interactions and disease relevance.
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