| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD3B2 |
| Uniprot No | P26439 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ |
| 分子量 | 68.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HSD3B2蛋白的参考文献示例，涵盖了蛋白表达、功能研究和疾病关联等方面的内容：
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### 1. **"Expression and Characterization of Recombinant Human 3β-Hydroxysteroid Dehydrogenase Type 2 (HSD3B2) in a Baculovirus System"**
**作者**: Smith J, et al.
**摘要**: 本研究利用杆状病毒表达系统成功表达并纯化了重组人HSD3B2蛋白。通过酶动力学分析发现，该蛋白在体外可将孕烯醇酮转化为孕酮，并依赖NAD+作为辅因子。研究还优化了表达条件以提高蛋白活性，为后续功能研究奠定了基础。
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### 2. **"Functional Analysis of HSD3B2 Mutations Causing Classic 3β-HSD Deficiency"**
**作者**: Miller WL, Auchus RJ.
**摘要**: 论文系统分析了先天性肾上腺皮质增生症(CAH)患者中发现的HSD3B2基因突变对酶活性的影响。通过在大肠杆菌中表达重组突变蛋白，发现部分突变导致酶活性显著降低或丧失，解释了类固醇合成障碍的分子机制。
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### 3. **"Crystal Structure of Human HSD3B2 Reveals Substrate Binding and Catalytic Mechanism"**
**作者**: Zhang Y, et al.
**摘要**: 本研究首次解析了重组人HSD3B2蛋白的X射线晶体结构，揭示了其与底物孕烯醇酮的结合模式及NAD+辅因子的相互作用位点。结构分析表明，活性中心的特定氨基酸残基对催化反应至关重要，为靶向药物设计提供了理论依据。
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### 4. **"Development of a Cell-Based Assay for High-Throughput Screening of HSD3B2 Inhibitors"**
**作者**: Lee C, et al.
**摘要**: 利用过表达重组HSD3B2的人胚胎肾细胞(HEK293)，研究者建立了一种高通量筛选模型，用于评估小分子化合物对HSD3B2活性的抑制作用。该方法可用于开发治疗多囊卵巢综合征(PCOS)或前列腺癌的潜在药物。
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**注意**：以上文献为示例性内容，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实存在的论文并核对作者及摘要信息。
**Recombinant human HSD3B2 protein** is a biologically engineered form of the 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2) enzyme, critical in steroid hormone biosynthesis. HSD3B2 catalyzes the oxidation and isomerization of Δ5-3β-hydroxysteroids (e.g., pregnenolone, 17α-hydroxypregnenolone, dehydroepiandrosterone) to Δ4-ketosteroids (e.g., progesterone, 17α-hydroxyprogesterone, androstenedione), a key step in producing glucocorticoids, mineralocorticoids, and sex hormones. This enzyme is predominantly expressed in steroidogenic tissues, including the adrenal glands, ovaries, and testes. Deficiencies in HSD3B2 due to genetic mutations cause congenital adrenal hyperplasia (CAH), leading to impaired cortisol/aldosterone synthesis, adrenal insufficiency, and androgen excess.
Recombinant HSD3B2 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, function, and mutations linked to CAH. It enables detailed enzymatic assays, inhibitor screening, and structural analysis (e.g., X-ray crystallography) to understand catalytic mechanisms and substrate specificity. Additionally, recombinant HSD3B2 serves as a tool for developing diagnostic assays and exploring therapeutic interventions for steroid-related disorders. Its production overcomes challenges in isolating native forms, ensuring consistent purity and activity for research and potential clinical applications.
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