| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS3ST2 |
| Uniprot No | Q9Y278 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDLGRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSNHSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDWYRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQTLSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGERLITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTHVQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE |
| 分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性参考文献示例，建议通过学术数据库(如PubMed、Google Scholar)以关键词“recombinant human HS3ST3”或“HS3ST2 sulfotransferase”检索真实文献：
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1. **标题**: *"Expression and Functional Analysis of Recombinant Human HS3ST2 in Viral Entry"*
**作者**: Lee S, et al.
**摘要**: 研究利用昆虫杆状病毒系统表达重组HS3ST2.证实其通过修饰宿主细胞表面硫酸肝素促进疱疹病毒(HSV-1)的吸附，为抗病毒治疗提供新靶点。
2. **标题**: *"Enzymatic Characterization of HS3ST2 in Heparan Sulfate Biosynthesis"*
**作者**: Wang Y, et al.
**摘要**: 报道重组人HS3ST2蛋白的体外酶动力学参数，揭示其对特定糖胺聚糖链的3-O-磺酸化选择性，并探讨其在肝素生物合成途径中的作用。
3. **标题**: *"HS3ST2 Modulates Axon Guidance via Sulfation Code Regulation"*
**作者**: Tanaka K, et al.
**摘要**: 使用重组HS3ST2处理神经元细胞，证明其通过改变硫酸肝素硫酸化模式影响Semaphorin信号传导，调控轴突导向。
4. **标题**: *"Structural Basis of Substrate Recognition by HS3ST2 Sulfotransferase"*
**作者**: Müller R, et al.
**摘要**: 解析重组HS3ST2蛋白的晶体结构，结合分子对接实验阐明其底物结合域，为酶活性的特异性调控提供结构依据。
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**注意**：以上文献为示例性质，实际文献需通过学术数据库检索。推荐关键词组合：`"HS3ST2" AND "recombinant"` 或 `"3-O-sulfotransferase" AND "heparan sulfate"`。
Heparan sulfate 3-O-sulfotransferase 2 (HS3ST2) is a member of the heparan sulfate sulfotransferase family, which catalyzes the transfer of sulfate groups to specific positions on heparan sulfate (HS) chains. These sulfation modifications critically determine HS interactions with proteins, influencing cell signaling, inflammation, and pathogen entry. The human HS3ST2 gene encodes an enzyme that specifically mediates 3-O-sulfation of glucosamine residues, a rare modification essential for HS-dependent biological activities like antithrombin III binding and herpes simplex virus (HSV-1) entry.
Recombinant human HS3ST2 protein is engineered using expression systems (e.g., mammalian or insect cells) to produce a purified, bioactive form for research. It serves as a tool to study HS biosynthesis, structure-function relationships, and roles in diseases such as cancer, viral infections, and fibrosis. Interest in HS3ST2 has grown due to its potential as a biomarker in inflammatory disorders and malignancies, including mesothelioma, where its overexpression correlates with poor prognosis. Additionally, its role in regulating growth factor signaling (e.g., Wnt, FGF) underscores therapeutic relevance in tissue repair and tumor microenvironments. Current challenges in HS3ST2 research include elucidating its substrate specificity in complex HS chains and translating structural insights into targeted therapies. Its recombinant form enables high-throughput assays for drug discovery and mechanistic studies, bridging gaps between glycobiology and disease pathophysiology.
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