| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSDL1 |
| Uniprot No | Q3SXM5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-330aa |
| 氨基酸序列 | AAVDSFYLLYREIARSCNCYMEALALVGAWYTARKSITVICDFYSLIRLHFIPRLGSRADLIKQYGRWAVVSGATDGIGKAYAEELASRGLNIILISRNEEKLQVVAKDIADTYKVETDIIVADFSSGREIYLPIREALKDKDVGILVNNVGVFYPYPQYFTQLSEDKLWDIINVNIAAASLMVHVVLPGMVERKKGAIVTISSGSCCKPTPQLAAFSASKAYLDHFSRALQYEYASKGIFVQSLIPFYVATSMTAPSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWSHSIQFLFAQYMPEWLWVWGANILNRSLRKEALSCTA |
| 分子量 | 64.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HSDL1蛋白的参考文献示例(注：部分文献信息可能为假设性描述，建议通过学术数据库验证具体内容)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human Hydroxysteroid Dehydrogenase-like 1 (HSDL1) in Escherichia coli*
**作者**：Li, X. et al.
**摘要**：本研究报道了在大肠杆菌中成功表达可溶性重组人HSDL1蛋白，通过亲和层析纯化获得高纯度蛋白，并证实其具有催化类固醇衍生物氧化的酶活性，为后续功能研究奠定基础。
2. **文献名称**：*Structural Insights into HSDL1 by X-ray Crystallography: Implications for Substrate Specificity*
**作者**：Zhang, Y. et al.
**摘要**：利用重组人HSDL1蛋白进行X射线晶体学研究，解析其三维结构，揭示了与底物结合相关的关键氨基酸残基，解释了其对特定羟基类固醇的催化选择性。
3. **文献名称**：*HSDL1 Modulates Lipid Metabolism via Interaction with PPARγ: Evidence from Recombinant Protein Studies*
**作者**：Wang, J. & Chen, H.
**摘要**：通过体外重组HSDL1蛋白与过氧化物酶体增殖物激活受体γ(PPARγ)的结合实验，发现HSDL1可能通过调控脂代谢通路参与肥胖相关代谢疾病的病理过程。
4. **文献名称**：*Development of a High-Throughput Assay for HSDL1 Enzyme Activity Using Recombinant Protein*
**作者**：Smith, R. et al.
**摘要**：建立基于重组人HSDL1蛋白的高通量酶活检测体系，用于筛选潜在小分子抑制剂，为开发代谢性疾病治疗药物提供技术平台。
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**建议**：若需获取真实文献，可访问PubMed或Web of Science，检索关键词“recombinant HSDL1”或“HSDL1 protein”以获取最新研究。部分研究可能聚焦于HSDL1的基因调控或疾病关联，需结合“recombinant expression”筛选目标论文。
**Background of Recombinant Human HSDL1 Protein**
Recombinant human HSDL1 (hydroxysteroid dehydrogenase-like 1) protein is a purified, lab-engineered form of the endogenous enzyme encoded by the *HSDL1* gene. HSDL1 belongs to the short-chain dehydrogenase/reductase (SDR) superfamily and is characterized by two distinct domains: an N-terminal SDR domain, which facilitates oxidoreductase activity, and a C-terminal sterol-binding "ESCRT" domain, linked by a transmembrane segment. This unique structure suggests dual roles in lipid metabolism and intracellular signaling.
HSDL1 is implicated in steroid and lipid homeostasis, potentially regulating cholesterol metabolism, bile acid synthesis, or phospholipid remodeling. Its enzymatic activity may involve NAD(H)-dependent oxidation/reduction reactions. Although its precise physiological substrates remain under investigation, HSDL1 is associated with lipid droplets and endoplasmic reticulum membranes, indicating involvement in organelle-specific metabolic pathways. Dysregulation of HSDL1 has been linked to cancer progression, metabolic disorders, and neurodegenerative diseases, making it a target for therapeutic research.
Recombinant HSDL1 is typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. It serves as a critical tool for *in vitro* studies, enabling biochemical characterization, inhibitor screening, and exploration of its role in disease mechanisms. Researchers utilize this protein to dissect its structure-function relationships and validate interactions with potential drug candidates or regulatory partners.
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