| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G4D |
| Uniprot No | Q86XP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-818aa |
| 氨基酸序列 | MESLSPGGPPGHPYQGEASTCWQLTVRVLEARNLRWADLLSEADPYVILQLSTAPGMKFKTKTLTDTSHPVWNEAFRFLIQSQVKNVLELSIYDEDSVTEDDICFKVLYDISEVLPGKLLRKTFSQSPQGEEELDVEFLMEETSDRPENLITNKVIVARELSCLDVHLDSTGSTAVVADQDKLELELVLKGSYEDTQTSFLGTASAFRFHYMAALETELSGRLRSSRSNGWNGDNSAGYLTVPLRPLTIGKEVTMDVPAPNAPGVRLQLKAEGCPEELAVHLGFNLCAEEQAFLSRRKQVVAKALKQALQLDRDLQEDEVPVVGIMATGGGARAMTSLYGHLLALQKLGLLDCVTYFSGISGSTWTMAHLYGDPEWSQRDLEGPIRYAREHLAKSKLEVFSPERLASYRRELELRAEQGHPTTFVDLWALVLESMLHGQVMDQKLSGQRAALERGQNPLPLYLSLNVKENNLETLDFKEWVEFSPYEVGFLKYGAFVPPELFGSEFFMGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEALQQTELYCRARGLPFPRVEPSPQDQHQPRECHLFSDPACPEAPILLHFPLVNASFKDHSAPGVQRSPAELQGGQVDLTGATCPYTLSNMTYKEEDFERLLRLSDYNVQTSQGAILQALRTALKHRTLEARPPRAQT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLA2G4D重组蛋白的3篇模拟参考文献及其摘要概述(注：以下内容为示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*"Characterization of recombinant human PLA2G4D and its role in epidermal lipid metabolism"*
**作者**：S. Ishida et al.
**摘要**：本研究成功表达并纯化了重组人PLA2G4D蛋白，发现其在体外表现出磷脂酶A2活性，且依赖钙离子激活。实验表明，PLA2G4D在表皮角质细胞中可能通过水解特定磷脂参与皮肤屏障相关脂质的代谢调控。
2. **文献名称**：*"PLA2G4D in psoriasis: Expression profiling and enzymatic activity of recombinant protein"*
**作者**：K. Yamamoto et al.
**摘要**：通过分析银屑病患者皮肤样本，发现PLA2G4D表达显著上调。利用重组PLA2G4D蛋白进行功能研究，发现其可通过释放花生四烯酸促进炎症反应，提示其可能作为银屑病治疗的潜在靶点。
3. **文献名称**：*"Structural insights into the catalytic mechanism of PLA2G4D through recombinant protein crystallization"*
**作者**：T. Nakamura & M. Tanaka
**摘要**：本研究解析了重组PLA2G4D蛋白的晶体结构，揭示了其活性位点与底物结合的关键氨基酸残基，为设计特异性抑制剂提供了结构基础。
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**提示**：PLA2G4D属于分泌型磷脂酶A2家族，现有研究多聚焦于其在皮肤屏障功能、炎症性疾病(如银屑病、特应性皮炎)中的作用及酶学机制。建议通过PubMed或Web of Science以“PLA2G4D recombinant”“phospholipase A2 IVD”为关键词检索最新文献。
**Background of PLA2G4D Recombinant Protein**
Phospholipase A2 Group IVD (PLA2G4D) is a calcium-dependent enzyme belonging to the phospholipase A2 (PLA2) superfamily, which hydrolyzes phospholipids at the *sn-2* position to release free fatty acids (e.g., arachidonic acid) and lysophospholipids. These products serve as precursors for bioactive lipid mediators, including prostaglandins and leukotrienes, which regulate inflammation, immunity, and cellular signaling. PLA2G4D is categorized under Group IV PLA2s, specifically the cytosolic PLA2 (cPLA2) subgroup, known for their role in intracellular lipid signaling.
The PLA2G4D gene encodes a protein with a conserved catalytic domain and calcium-binding motifs critical for its enzymatic activity and membrane association. Unlike ubiquitously expressed cPLA2 isoforms (e.g., PLA2G4A), PLA2G4D exhibits restricted expression, primarily in differentiated epidermal keratinocytes and certain immune cells, suggesting specialized roles in skin barrier homeostasis and localized inflammatory responses. Studies link PLA2G4D to pathologies such as psoriasis, atopic dermatitis, and cancer, where its dysregulation may alter lipid metabolism or promote pro-inflammatory signaling.
Recombinant PLA2G4D is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its recombinant form retains enzymatic activity, allowing researchers to investigate substrate specificity, inhibitor screening, and mechanistic roles in disease models. Recent interest in PLA2G4D stems from its potential as a therapeutic target, particularly in inflammatory skin disorders, and its unique tissue-specific expression pattern, which may reduce off-target effects in drug development. Advances in structural biology and lipidomics continue to unravel its regulatory mechanisms and interaction networks, positioning PLA2G4D as a key player in lipid-mediated cellular processes.
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