| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSN2 |
| Uniprot No | Q9H4A3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MYELLVLFMLIQPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQVIGKLPQLF |
| 分子量 | 73.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HSN2蛋白的参考文献示例(注：HSN2相关研究较少，可能与SPTLC1或相关神经病变蛋白研究重叠，以下是模拟参考格式)：
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1. **文献名称**: *Cloning and functional analysis of HSN2 in sensory neuron maintenance*
**作者**: Shekarabi M, et al.
**摘要**: 研究报道了人HSN2基因的克隆及其编码蛋白的杆状病毒表达系统构建，发现重组HSN2蛋白参与调控神经鞘脂代谢，突变导致感觉神经元退化。
2. **文献名称**: *Recombinant human HSN2 protein rescues neuropathy in cellular models*
**作者**: Penno A, et al.
**摘要**: 通过哺乳动物细胞表达重组人HSN2蛋白，证实其通过调节丝氨酸棕榈酰转移酶(SPT)复合物活性，可逆转HSAN患者细胞模型的代谢缺陷。
3. **文献名称**: *Structural insights into HSN2/SPTLC1 complex by cryo-EM*
**作者**: Wang Y, et al.
**摘要**: 利用冷冻电镜解析了重组HSN2蛋白与SPTLC1的复合物结构，揭示了其介导神经鞘脂合成的分子机制，为药物开发提供靶点。
4. **文献名称**: *Expression optimization of recombinant HSN2 in E. coli for enzymatic assays*
**作者**: Zhang L, et al.
**摘要**: 优化了大肠杆菌中重组HSN2蛋白的可溶性表达与纯化方法，验证其在体外酶活测定中的功能，推动高通量抑制剂筛选研究。
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*提示*：HSN2(即SPTLC1)相关研究多聚焦于其基因突变与神经病变的关联，实际文献中"重组HSN2蛋白"的提法较少，建议结合“SPTLC1 recombinant”或“hereditary sensory neuropathy protein”等关键词检索。如需真实文献，可通过PubMed检索对应主题。
Recombinant human HSN2 protein is a genetically engineered protein derived from the human HSN2 gene, which plays a critical role in neuronal function and sensory perception. HSN2. also known as *WNK1* (With No Lysine Kinase 1)-associated protein, is implicated in maintaining peripheral sensory neurons, particularly those involved in pain and temperature sensation. Mutations in the HSN2 gene are linked to hereditary sensory and autonomic neuropathy type II (HSAN2), a rare genetic disorder characterized by progressive loss of sensory neurons, leading to insensitivity to pain, ulcers, and tissue damage.
The recombinant HSN2 protein is typically produced using expression systems like Escherichia coli or mammalian cells, enabling researchers to study its structure, interaction partners, and molecular mechanisms. It serves as a vital tool for investigating the pathophysiology of HSAN2 and exploring therapeutic strategies, such as gene therapy or protein replacement. Studies suggest HSN2 interacts with cytoskeletal components and ion channels, influencing neuronal development and signaling. Its recombinant form facilitates in vitro and in vivo experiments to dissect these pathways, aiding drug discovery and diagnostics. Additionally, it supports research on broader neuropathic conditions, offering insights into sensory neuron maintenance and degeneration mechanisms.
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