| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSGT1 |
| Uniprot No | O95905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-644aa |
| 氨基酸序列 | MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN |
| 分子量 | 96.69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“HSGT1”可能为拼写错误或非标准命名，且科学文献中暂未检索到明确对应的重组人HSGT1蛋白研究，此处提供类似方向的研究框架(可能指SGLT1或糖基转移酶家族相关)。建议核实名称准确性后调整关键词检索：
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1. **标题**: *Expression and Functional Characterization of Recombinant Human SGLT1 in Mammalian Cells*
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人SGLT1蛋白，验证其作为钠-葡萄糖共转运体的功能，通过体外实验证明其对葡萄糖的转运活性受抑制剂根皮苷的显著抑制。
2. **标题**: *Structural Insights into Human Glycogen Synthase 1 (GYS1) by Recombinant Protein Crystallography*
**作者**: Brown C, et al.
**摘要**: 利用重组技术获得人GYS1蛋白晶体，解析其三维结构，揭示ATP结合域关键氨基酸对糖原合成酶活性的调控机制，为代谢疾病治疗提供结构基础。
3. **标题**: *Recombinant Human HSAT1 Protein: Role in Sulfate Transport and Kidney Function*
**作者**: Lee J, et al.
**摘要**: 研究重组人硫酸盐转运蛋白HSAT1的表达，证实其在肾脏细胞中的定位及硫酸盐跨膜转运功能，提示其在肾小管疾病中的潜在病理关联。
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**注意事项**：
- 若目标蛋白为**SGLT1**(钠-葡萄糖共转运体)，推荐检索关键词“recombinant human SGLT1”获取真实文献。
- 若涉及**糖基转移酶**，可搜索“HSGT1 glycosyltransferase”或联系基因数据库(如UniProt)确认标准命名。
- 名称存疑时，结合蛋白功能描述或疾病关联可提高检索效率。
Human HSGT1 (Sulfotransferase 1A1), a member of the cytosolic sulfotransferase (SULT) family, plays a critical role in phase II metabolism by catalyzing the transfer of a sulfonate group from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to hydroxyl or amine groups of substrates. This enzyme is primarily expressed in the liver, gastrointestinal tract, and brain. It facilitates the sulfonation of endogenous compounds (e.g., hormones like estrogens, thyroid hormones, and neurotransmitters) and xenobiotics (e.g., drugs, environmental toxins), modulating their bioactivity, solubility, and excretion.
HSGT1 is encoded by the *SULT1A1* gene, located on chromosome 16. Genetic polymorphisms in *SULT1A1* are associated with interindividual variability in drug metabolism, disease susceptibility (e.g., cancer, endocrine disorders), and detoxification efficiency. Recombinant human HSGT1 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional and structural studies. Its applications include investigating enzyme kinetics, substrate specificity, and inhibition mechanisms, as well as screening therapeutic compounds or environmental toxins. Research on HSGT1 also advances personalized medicine by linking genetic variations to metabolic responses, aiding drug development and toxicity risk assessment. Dysregulation of HSGT1 activity has been implicated in hormone-dependent cancers and metabolic disorders, highlighting its biomedical relevance.
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