| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSFY1 |
| Uniprot No | Q96LI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-401aa |
| 氨基酸序列 | MAHVSSETQDVSPKDELTASEASTRSPLCEHTFPGDSDLRSMIEEHAFQVLSQGSLLESPSYTVCVSEPDKDDDFLSLNFPRKLWKIVESDQFKSISWDENGTCIVINEELFKKEILETKAPYRIFQTDAIKSFVRQLNLYGFSKIQQNFQRSAFLATFLSEEKESSVLSKLKFYYNPNFKRGYPQLLVRVKRRIGVKNASPISTLFNEDFNKKHFRAGANMENHNSALAAEASEESLFSASKNLNMPLTRESSVRQIIANSSVPIRSGFPPPSPSTSVGPSEQIATDQHAILNQLTTIHMHSHSTYMQARGHIVNFITTTTSQYHIISPLQNGYFGLTVEPSAVPTRYPLVSVNEAPYRNMLPAGNPWLQMPTIADRSAAPHSRLALQPSPLDKYHPNYN |
| 分子量 | 71.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HSFY1蛋白的参考文献示例(注：文献为模拟示例，实际文献可能需要通过学术数据库确认)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human HSFY1 Protein*
**作者**：Zhang L, et al.
**摘要**：该研究成功克隆并表达了重组人HSFY1蛋白，利用大肠杆菌表达系统进行纯化。通过体外实验验证了HSFY1与特定DNA序列的相互作用，揭示了其在精子发生中潜在的基因调控功能。
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2. **文献名称**：*HSFY1 Deficiency Impairs Spermatogenesis via Disrupting Histone-to-Protamine Transition*
**作者**：Sato Y, et al.
**摘要**：研究者通过构建HSFY1基因敲除小鼠模型，发现其雄性不育表型。利用重组HSFY1蛋白进行功能恢复实验，证实其在组蛋白-鱼精蛋白转换过程中的关键作用，为男性不育机制提供了新见解。
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3. **文献名称**：*Structural and Functional Analysis of HSFY1 in Male Germ Cell Development*
**作者**：Wang X, et al.
**摘要**：该研究解析了重组HSFY1蛋白的三维结构，结合CRISPR/Cas9技术筛选了HSFY1的相互作用蛋白，发现其与染色体重塑复合物的关联，进一步阐明其在减数分裂中的调控机制。
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4. **文献名称**：*Mutations in HSFY1 Correlate with Azoospermia in Human Patients*
**作者**：Liu R, et al.
**摘要**：通过对无精症患者的基因测序，发现HSFY1突变位点。体外表达突变型重组HSFY1蛋白后，观察到其DNA结合能力显著降低，提示突变可能导致精子发生障碍。
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**说明**：以上文献为示例性内容，实际研究需参考真实发表的论文。建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索关键词如“recombinant HSFY1”、“HSFY1 protein function”等获取最新进展。
**Background of Recombinant Human HSFY1 Protein**
HSFY1 (Heat Shock Transcription Factor, Y-linked 1) is a member of the HSF family, encoded by genes on the Y chromosome. It is predominantly expressed in male germ cells, particularly during spermatogenesis, suggesting a role in regulating testis-specific gene expression and spermatogenic processes. Structurally, HSFY1 contains conserved heat shock factor (HSF)-type DNA-binding domains, enabling interaction with heat shock elements (HSEs) to modulate transcriptional activity.
As a Y-chromosomal protein, HSFY1 has garnered interest in male infertility research, as deletions or mutations in its coding regions are associated with non-obstructive azoospermia (NOA) and impaired sperm production. Recombinant human HSFY1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It enables *in vitro* investigations into its DNA-binding properties, protein-protein interactions (e.g., with other HSF members), and roles in stress response or germ cell development.
Current research focuses on clarifying HSFY1's molecular mechanisms in spermatogenesis, its potential diagnostic utility in infertility, and its broader regulatory functions beyond male reproduction. Challenges include elucidating its tissue-specific regulatory networks and redundancy with autosomal HSF homologs. Recombinant HSFY1 serves as a vital tool for exploring these biological questions and developing therapeutic strategies for Y-linked reproductive disorders.
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