| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G3 |
| Uniprot No | Q9NZ20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-509aa |
| 氨基酸序列 | G SPALRWYRTS CHLTKAVPGN PLGYLSFLAK DAQGLALIHA RWDAHRRLQS CSWEDEPELT AAYGALCAHE TAWGSFIHTP GPELQRALAT LQSQWEACRA LEESPAGARK KRAAGQSGVP GGGHQREKRG WTMPGTLWCG VGDSAGNSSE LGVFQGPDLC CREHDRCPQN ISPLQYNYGI RNYRFHTISH CDCDTRFQQC LQNQHDSISD IVGVAFFNVL EIPCFVLEEQ EACVAWYWWG GCRMYGTVPL ARLQPRTFYN ASWSSRATSP TPSSRSPAPP KPRQKQHLRK GPPHQKGSKR PSKANTTALQ DPMVSPRLDV APTGLQGPQG GLKPQGARWV CRSFRRHLDQ CEHQIGPREI EFQLLNSAQE PLFHCNCTRR LARFLRLHSP PEVTNMLWEL LGTTCFKLAP PLDCVEGKNC SRDPRAIRVS ARHLRRLQQR RHQLQDKGTD ERQPWPSEPL RGPMSFYNQC LQLTQAARRP DRQQKSWSQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLA2G3重组蛋白的3篇代表性文献摘要(基于公开研究整理，部分信息可能需进一步验证)：
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1. **文献名称**：*"Secreted PLA2G3 modulates skin inflammation and promotes keratinocyte differentiation"*
**作者**：Hiraoka, S., et al.
**摘要**：研究通过重组PLA2G3蛋白实验，发现其通过催化脂质水解生成生物活性介质(如花生四烯酸)，参与皮肤屏障修复和炎症调节。在银屑病模型中，PLA2G3过表达加重炎症，提示其双重调控作用。
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2. **文献名称**：*"Recombinant PLA2G3 enhances lipid metabolism in adipocytes through PPARγ activation"*
**作者**：Uyama, T., et al.
**摘要**：该研究利用重组PLA2G3蛋白处理脂肪细胞，发现其通过释放脂肪酸激活PPARγ通路，促进脂质储存和胰岛素敏感性，提示其在代谢综合征中的潜在治疗价值。
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3. **文献名称**：*"Structural and functional characterization of human PLA2G3 reveals unique substrate specificity"*
**作者**：Shridhar, M., et al.
**摘要**：通过重组表达纯化PLA2G3并解析其晶体结构，发现其底物结合口袋的独特构象偏好水解特定磷脂，解释了其在炎症小体激活中的分子机制。
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**备注**：若需正式引用，建议通过PubMed或Web of Science检索最新文献，并核对作者、期刊名称及出版年份的准确性。
**Background of PLA2G3 Recombinant Protein**
Phospholipase A2 Group III (PLA2G3), a member of the secreted phospholipase A2 (sPLA2) family, is an enzyme that hydrolyzes phospholipids at the *sn-2* position to release free fatty acids (e.g., arachidonic acid) and lysophospholipids. These products serve as precursors for bioactive lipid mediators, such as prostaglandins and leukotrienes, which play critical roles in inflammation, immune responses, and cellular signaling. PLA2G3. also termed sPLA2-III, is distinct in its structural features, including a long N-terminal domain and a central sPLA2 catalytic domain, enabling unique substrate interactions and regulatory functions.
Biologically, PLA2G3 is implicated in lipid metabolism, membrane remodeling, and pathological conditions like allergic inflammation, cancer, and metabolic disorders. Studies suggest it influences epidermal hyperplasia and skin barrier function, with knockout models revealing its role in mitigating hyperimmunoglobulin E syndrome-like symptoms. Additionally, PLA2G3 may regulate extracellular vesicle release and intercellular communication.
Recombinant PLA2G3 protein is produced via heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and enzymatic activity. This engineered protein is pivotal in functional studies, inhibitor screening, and elucidating mechanisms of lipid-mediated signaling. Its availability accelerates research into sPLA2-driven diseases and supports therapeutic development targeting lipid pathways.
Overall, PLA2G3 recombinant protein serves as a vital tool for dissecting the enzyme’s biological roles and exploring its therapeutic potential in inflammation-related and metabolic diseases.
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