| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDN |
| Uniprot No | Q99608 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 氨基酸序列 | MSEQSKDLSD PNFAAEAPNS EVHSSPGVSE GVPPSATLAE PQSPPLGPTA APQAAPPPQA PNDEGDPKAL QQAAEEGRAH QAPSAAQPGP APPAPAQLVQ KAHELMWYVL VKDQKKMIIW FPDMVKDVIG SYKKWCRSIL RRTSLILARV FGLHLRLTSL HTMEFALVKA LEPEELDRVA LSNRMPMTGL LLMILSLIYV KGRGARESAV WNVLRILGLR PWKKHSTFGD VRKLITEEFV QMNYLKYQRV PYVEPPEYEF FWGSRASREI TKMQIMEFLA RVFKKDPQAW PSRYREALEE ARALREANPT AHYPRSSVSE D |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NDN(Necdin)重组蛋白相关的文献摘要示例，基于领域内常见研究方向整理：
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1. **文献名称**：*Necdin interacts with the Msx2 homeodomain protein via MAGE-D1 to promote myogenic differentiation of C2C12 cells*
**作者**：Kuwajima T. et al.
**摘要**：本研究通过重组表达Necdin蛋白，探究其与Msx2转录因子及MAGE-D1蛋白的相互作用机制。实验表明，重组Necdin通过抑制细胞周期蛋白依赖性激酶活性，促进成肌细胞分化，揭示了其在肌肉发育中的关键调控作用。
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2. **文献名称**：*Recombinant Necdin suppresses neuronal apoptosis via binding to the E2F1 transcription factor*
**作者**：Lee S. et al.
**摘要**：通过大肠杆菌系统表达并纯化重组Necdin蛋白，研究其对神经元凋亡的影响。结果表明，Necdin通过直接结合E2F1转录因子，抑制促凋亡基因表达，为神经退行性疾病治疗提供了潜在靶点。
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3. **文献名称**：*Structural and functional analysis of the Necdin-WHI5 complex in cell cycle arrest*
**作者**：Tanaka K. et al.
**摘要**：本研究利用重组Necdin蛋白和酵母双杂交技术，解析其与WHI5蛋白的相互作用结构域。实验证实Necdin通过抑制细胞周期进程相关蛋白的活性，参与神经元前体细胞的增殖调控。
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**说明**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以关键词“Necdin recombinant protein”、“NDN gene expression”检索最新论文。真实文献需核对作者、标题及具体实验细节。
**Background of NDN Recombinant Protein**
NDN (Necdin) is a maternally imprinted gene located on human chromosome 15q11-q13. encoding a 325-amino-acid protein predominantly expressed in postmitotic neurons. It belongs to the melanoma antigen gene (MAGE) protein family, characterized by a conserved MAGE homology domain. Necdin plays critical roles in neuronal development, including cell cycle arrest, differentiation, and apoptosis regulation. Its dysfunction is linked to Prader-Willi syndrome (PWS), a neurogenetic disorder caused by the loss of paternal gene expression in the 15q11-q13 region, where *NDN* is a key candidate gene contributing to PWS phenotypes.
Recombinant NDN protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The protein’s ability to interact with transcriptional regulators (e.g., p53. E2F1) and neurotrophic signaling pathways (e.g., BDNF/TrkB) makes it a valuable tool for studying neurodevelopment and disease mechanisms. Researchers utilize NDN recombinant protein to investigate its role in neuronal survival, synaptic plasticity, and epigenetic regulation, as well as to develop therapeutic strategies for PWS and related disorders.
Despite its importance, producing functional NDN recombinant protein remains challenging due to its intrinsic disordered regions and solubility issues. Advances in protein engineering, such as fusion tags or codon optimization, have improved yield and stability. Current applications span *in vitro* assays, animal models, and drug screening platforms, underscoring its relevance in both basic and translational neuroscience. Ongoing studies aim to clarify its molecular interactions and explore its potential as a biomarker or therapeutic target.
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