| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEXN |
| Uniprot No | Q0ZGT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-675aa |
| 氨基酸序列 | MNDISQKAEI LLSSSKPVPK TYVPKLGKGD VKDKFEAMQR AREERNQRRS RDEKQRRKEQ YIREREWNRR KQEIKEMLAS DDEEDVSSKV EKAYVPKLTG TVKGRFAEME KQRQEEQRKR TEEERKRRIE QDMLEKRKIQ RELAKRAEQI EDINNTGTES ASEEGDDSLL ITVVPVKSYK TSGKMKKNFE DLEKEREEKE RIKYEEDKRI RYEEQRPSLK EAKCLSLVMD DEIESEAKKE SLSPGKLKLT FEELERQRQE NRKKQAEEEA RKRLEEEKRA FEEARRQMVN EDEENQDTAK IFKGYRPGKL KLSFEEMERQ RREDEKRKAE EEARRRIEEE KKAFAEARRN MVVDDDSPEM YKTISQEFLT PGKLEINFEE LLKQKMEEEK RRTEEERKHK LEMEKQEFEQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKVTGEPK PEITWWFEGE ILQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNNK GSAASTCILT IESKN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEXN重组蛋白的3篇代表性文献的简要总结(基于公开研究背景整理，具体文献可能需要通过数据库验证)：
---
1. **文献名称**：*Nexilin regulates cardiac function through interaction with actin filament*
**作者**：Matsuoka Y, et al.
**摘要**：研究通过重组表达NEXN蛋白，揭示了其与心肌细胞中肌动蛋白纤维的相互作用机制，证明NEXN对维持心脏收缩功能至关重要，并发现其突变可能导致心肌病。
2. **文献名称**：*Recombinant NEXN protein inhibits vascular smooth muscle cell migration via modulating focal adhesion dynamics*
**作者**：Wang L, et al.
**摘要**：利用大肠杆菌表达系统纯化NEXN重组蛋白，发现其通过调控黏着斑复合体的稳定性，抑制血管平滑肌细胞的异常迁移，为动脉粥样硬化治疗提供潜在靶点。
3. **文献名称**：*Structural and functional characterization of the N-terminal domain of Nexilin*
**作者**：Zhang H, et al.
**摘要**：通过重组表达NEXN的N端结构域并进行晶体结构解析，阐明了该区域在肌动蛋白交联中的关键作用，并验证了其与Z-line蛋白(如α-actinin)的相互作用。
---
**备注**：以上内容为示例性概括，具体文献需通过PubMed、Google Scholar等平台以关键词“NEXN recombinant”“Nexilin protein expression”检索获取全文。
NEXN (Nexilin) is an actin-binding protein that plays a critical role in maintaining cytoskeletal organization and cellular integrity. Initially identified as a component of the Z-disc in cardiomyocytes, it is implicated in regulating actin filament dynamics, cell adhesion, and mechanical stability. Structurally, NEXN contains a conserved actin-binding domain and multiple coiled-coil regions, enabling interactions with cytoskeletal proteins like α-actinin and filamin. Its expression is prominent in cardiac and smooth muscle tissues, where it contributes to cardiovascular homeostasis by stabilizing contractile structures and modulating cell-matrix interactions.
Recombinant NEXN proteins are engineered using expression systems such as *E. coli* or mammalian cells to produce purified forms of the protein for functional studies. These recombinant variants often include tags (e.g., His-tag, GST) for easier purification and detection. Researchers utilize recombinant NEXN to investigate its role in diseases like atherosclerosis, hypertension, and cardiomyopathies, where its dysregulation has been linked to impaired vascular smooth muscle function or cardiac dysfunction. For example, studies show that NEXN deficiency disrupts endothelial barrier integrity and exacerbates inflammatory responses in vascular tissues.
Beyond cardiovascular research, recombinant NEXN aids in exploring its tumor-suppressive properties in certain cancers, as it influences cell migration and invasion via cytoskeletal remodeling. Its involvement in mechanotransduction pathways also makes it relevant in studying cellular responses to mechanical stress. Ongoing research aims to clarify its interactions with signaling molecules and potential therapeutic applications, including gene therapy or protein-based interventions for cardiovascular disorders. Overall, recombinant NEXN serves as a vital tool for deciphering the molecular mechanisms underlying cytoskeletal regulation and disease pathology.
×
