| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKAP |
| Uniprot No | Q8N5F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS GDRNGLTHQL GGLSQGSRNQ SYRSRSRSRS RERPSAPRGI PFASASSSVY YGSYSRPYGS DKPWPSLLDK EREESLRQKR LSERERIGEL GAPEVWGLSP KNPEPDSDEH TPVEDEEPKK STTSASTSEE EKKKKSSRSK ERSKKRRKKK SSKRKHKKYS EDSDSDSDSE TDSSDEDNKR RAKKAKKKEK KKKHRSKKYK KKRSKKSRKE SSDSSSKESQ EEFLENPWKD RTKAEEPSDL IGPEAPKTLT SQDDKPLNYG HALLPGEGAA MAEYVKAGKR IPRRGEIGLT SEEIASFECS GYVMSGSRHR RMEAVRLRKE NQIYSADEKR ALASFNQEER RKRENKILAS FREMVYRKTK GKDDK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKAP重组蛋白的3篇文献示例(内容为模拟概括，仅供参考)：
1. **文献名称**：NKAP regulates T cell development by controlling histone methylation
**作者**：Xiong Y, et al.
**摘要**：研究通过重组NKAP蛋白的体外实验，揭示其通过介导组蛋白H3K9甲基化修饰调控T细胞分化，并证明其缺失导致免疫缺陷。
2. **文献名称**：Structural insights into NKAP function in transcriptional repression
**作者**：Chen L, et al.
**摘要**：利用重组NKAP蛋白进行晶体结构解析，发现其C端结构域与HDAC3复合物相互作用，阐明其通过表观遗传机制抑制NF-κB通路的分子基础。
3. **文献名称**：NKAP promotes oncogenesis via stabilization of MYC mRNA in colorectal cancer
**作者**：Wang H, et al.
**摘要**：通过重组蛋白互作实验证实NKAP与RNA结合蛋白相互作用，增强MYC mRNA稳定性，促进结直肠癌细胞增殖，提示其作为癌症治疗靶点的潜力。
注：以上文献信息为模拟创作，实际引用需以真实文献为准。建议通过PubMed等数据库检索最新研究。
**Background of NKAP Recombinant Protein**
NKAP (NF-κB-activating protein) is a highly conserved nuclear protein involved in diverse cellular processes, including immune regulation, transcriptional control, and chromatin remodeling. Initially identified as a coactivator of NF-κB signaling, NKAP interacts with components of the Notch signaling pathway and the Death-Inducible Obliteration Complex (DISC), influencing T-cell development, immune homeostasis, and cell survival. Structurally, NKAP contains a conserved N-terminal domain critical for protein-protein interactions and a C-terminal region implicated in transcriptional regulation.
Recombinant NKAP protein is engineered using genetic cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This purified protein serves as a vital tool for studying NKAP's molecular mechanisms, such as its role in T-cell maturation, cytokine production, and epigenetic regulation via interactions with histone deacetylases (HDACs). Dysregulation of NKAP is linked to immune disorders, cancers, and developmental defects, underscoring its biological significance.
In research, NKAP recombinant protein is utilized in *in vitro* binding assays, structural studies, and functional analyses to dissect pathways in immunity and disease. Its application extends to drug discovery, particularly in targeting NF-κB or Notch-associated pathologies. By enabling precise biochemical characterization, recombinant NKAP accelerates understanding of its dual roles as a transcriptional regulator and immune modulator, offering potential therapeutic avenues for immune-related and malignant conditions.
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