| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL21 |
| Uniprot No | Q7Z2W9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-205 aa |
| 活性数据 | N SQSTSYLPGY VPKTSLSSPP WPEVVLPDPV EETRHHAEVV KKVNEMIVTG QYGRLFAVVH FASRQWKVTS EDLILIGNEL DLACGERIRL EKVLLVGADN FTLLGKPLLG KDLVRVEATV IEKTESWPRI IMRFRKRKNF KKKRIVTTPQ TVLRINSIEI APCLL |
| 分子量 | 22.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL21蛋白的3篇参考文献概览：
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1. **文献名称**: *MRPL21 is a novel mitochondrial protein essential for mitochondrial respiration*
**作者**: Smith A, et al.
**摘要**: 本研究首次报道人源MRPL21蛋白在线粒体核糖体组装中的关键作用。通过基因敲除实验，发现MRPL21缺失导致线粒体翻译功能受损、氧化磷酸化复合物合成减少，并引发细胞能量代谢障碍，证实其对维持线粒体呼吸链功能至关重要。
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2. **文献名称**: *Crystal structure of the human mitochondrial ribosomal protein MRPL21 reveals a conserved functional domain*
**作者**: Chen L, et al.
**摘要**: 研究人员成功表达并纯化了重组人MRPL21蛋白，通过X射线晶体学解析其三维结构，揭示了一个与细菌核糖体蛋白同源的保守结构域。该结构为理解MRPL21在线粒体翻译中的分子机制提供了结构基础。
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3. **文献名称**: *Dysregulation of MRPL21 expression correlates with hepatocellular carcinoma progression*
**作者**: Wang Y, et al.
**摘要**: 本文探讨了MRPL21在肝癌中的异常表达。实验显示肝癌组织中MRPL21 mRNA和蛋白水平显著下调，其低表达与患者预后不良相关。体外研究表明，重组MRPL21过表达可抑制肝癌细胞增殖并诱导凋亡，提示其可能的肿瘤抑制功能。
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注：以上文献为虚拟示例，实际研究需通过PubMed或Google Scholar检索具体论文。建议使用关键词 "MRPL21" + "mitochondrial ribosome" + "recombinant expression" 获取最新进展。
Mitochondrial Ribosomal Protein L21 (MRPL21) is a nuclear-encoded component of the mitochondrial large ribosomal subunit (39S), essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, possess their own translational machinery to synthesize core subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP generation. MRPL21 plays a structural and functional role in assembling and stabilizing the 39S subunit, ensuring proper mitochondrial ribosome integrity and translation efficiency. Dysregulation of mitochondrial ribosome proteins, including MRPL21. has been linked to impaired mitochondrial respiration, cellular energy deficits, and diseases such as neurodegenerative disorders, metabolic syndromes, and cancers.
The recombinant human MRPL21 protein is typically expressed in vitro using bacterial or eukaryotic systems, enabling studies on its molecular interactions, structural contributions to ribosome assembly, and pathological mechanisms. Research utilizing recombinant MRPL21 aids in elucidating its role in mitochondrial dysfunction-related diseases and screening for therapeutic agents targeting mitochondrial translation. Its study also contributes to understanding evolutionary conservation of ribosomal components and tissue-specific expression patterns in health and disease.
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