| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL4 |
| Uniprot No | Q9BYD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263 aa |
| 活性数据 | MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC |
| 分子量 | 54.67 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL4蛋白的3篇代表性文献摘要(注意：实际文献可能需要通过学术数据库验证)：
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1. **文献名称**：*Mitochondrial ribosomal protein L4 regulates translation and cell growth in Drosophila*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了MRPL4在线粒体翻译中的关键作用，通过重组人MRPL4蛋白回补实验，发现其缺失导致线粒体核糖体组装异常，影响呼吸链复合体活性及细胞增殖。
2. **文献名称**：*Structural insights into the role of MRPL4 in mitoribosome biogenesis*
**作者**：Brown A, et al.
**摘要**：利用重组表达的MRPL4蛋白进行冷冻电镜分析，解析了其在线粒体核糖体大亚基组装中的构象变化，揭示了突变导致的能量代谢疾病分子机制。
3. **文献名称**：*MRPL4 mutations cause a novel mitochondrial cardiomyopathy syndrome*
**作者**：Lee S, et al.
**摘要**：通过患者基因测序发现MRPL4突变，体外实验显示重组突变型MRPL4蛋白破坏线粒体蛋白合成，导致心肌病表型，提示其为潜在治疗靶点。
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**注意**：以上为示例性内容，实际文献需通过PubMed或Google Scholar以“MRPL4”、“mitochondrial ribosome”、“recombinant human MRPL4”等关键词检索获取。近年结构生物学与疾病机制研究可能更相关。
**Background of Recombinant Human MRPL4 Protein**
Mitochondrial Ribosomal Protein L4 (MRPL4) is a nuclear-encoded component of the large subunit (39S) of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Human MRPL4. located on chromosome 19. is critical for assembling functional ribosomes within mitochondria, enabling the translation of mitochondrial DNA-encoded genes involved in oxidative phosphorylation (OXPHOS). This protein plays a role in maintaining structural integrity of the ribosome and mediating interactions with mitochondrial rRNA or other ribosomal proteins.
Recombinant MRPL4 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, leveraging its purified form to investigate mitochondrial translation mechanisms, ribosome biogenesis, and energy metabolism. Dysregulation of MRPL4 has been linked to mitochondrial disorders, cancer, and metabolic syndromes due to impaired OXPHOS, which disrupts ATP production and promotes cellular dysfunction. Research on recombinant MRPL4 aids in elucidating its structural role, interactions within the ribosomal complex, and potential as a biomarker or therapeutic target. Its study also contributes to understanding diseases caused by mutations in mitochondrial ribosomal proteins or rRNA, highlighting its importance in cellular energy homeostasis and disease pathology.
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