| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS10 |
| Uniprot No | P82664 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201 aa |
| 活性数据 | MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLDS YEYFAVLAAK ELGISIKVHE PPRKIERFTL LQSVHIYKKH RVQYEMRTLY RCLELEHLTG STADVYLEYI QRNLPEGVAM EVTKTQLEQL PEHIKEPIWE TLSEEKEESK S |
| 分子量 | 22.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人线粒体核糖体蛋白S10(MRPS10)的参考文献及其简要摘要：
1. **文献名称**: *Mitochondrial ribosomal protein S10 is a pentatricopeptide repeat protein*
**作者**: Smits P, et al. (2013)
**摘要**: 揭示了MRPS10作为线粒体核糖体组成蛋白的功能，发现其具备五肽重复结构域，可能在RNA结合或核糖体组装中起作用，并讨论了其突变与线粒体功能异常的关联。
2. **文献名称**: *MRPS10 mutation associated with sideroblastic anemia and mitochondrial complex III deficiency*
**作者**: Riley LG, et al. (2016)
**摘要**: 报道了MRPS10基因突变导致线粒体复合物III缺乏和铁粒幼细胞性贫血的病例，表明该蛋白在血红素合成与能量代谢中的关键作用。
3. **文献名称**: *MRPS10 binds to the mitochondrial genome and regulates ROS production*
**作者**: Wang K, et al. (2019)
**摘要**: 发现MRPS10直接与线粒体DNA结合，通过调节核糖体组装影响氧化磷酸化效率，其缺失导致活性氧(ROS)累积和细胞凋亡加剧。
4. **文献名称**: *Proteomic analysis identifies MRPS10 as a biomarker for hepatocellular carcinoma*
**作者**: Chen J, et al. (2021)
**摘要**: 通过蛋白质组学分析发现MRPS10在肝癌组织中显著高表达，提示其可作为癌症代谢重编程的标志物，并可能成为治疗靶点。
**注意事项**：以上文献标题和内容为模拟示例，建议通过PubMed、Google Scholar或Web of Science以关键词“MRPS10”、“mitochondrial ribosome”、“disease”进一步检索最新研究。
**Recombinant Human MRPS10 Protein: Background**
Mitochondrial Ribosomal Protein S10 (MRPS10) is a key component of the small subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translational machinery to synthesize proteins encoded by mitochondrial DNA (mtDNA), particularly subunits of the electron transport chain complexes crucial for oxidative phosphorylation. MRPS10. encoded by nuclear DNA, is imported into mitochondria and plays a structural and functional role in assembling the 28S ribosomal subunit, facilitating accurate translation of mtDNA-encoded mRNAs.
Dysregulation of MRPS10 has been linked to mitochondrial disorders, metabolic diseases, and cancer, reflecting its importance in maintaining mitochondrial homeostasis. Recombinant human MRPS10 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to study its molecular interactions, structure, and role in mitochondrial dysfunction. It enables *in vitro* investigations, such as binding assays, enzyme kinetics, or cellular rescue experiments in MRPS10-deficient models.
Additionally, recombinant MRPS10 serves as an antigen for antibody production, aiding in disease diagnostics and biomarker research. Its study contributes to understanding mitochondrial ribosome assembly, pathogenic mutations, and potential therapeutic targets for diseases linked to mitochondrial translation defects.
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