| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS11 |
| Uniprot No | P82912 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194 aa |
| 活性数据 | MQAVRNAGSRFLRSWTWPQTAGRVVARTPAGTICTGARQLQDAAAKQKVEQNAAPSHTKFSIYPPIPGEESSLRWAGKKFEEIPIAHIKASHNNTQIQVVSASNEPLAFASCGTEGFRNAKKGTGIAAQTAGIAAAARAKQKGVIHIRVVVKGLGPGRLSAMHGLIMGGLEVISITDNTPIPHNGCRPRKARKL |
| 分子量 | 47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS11蛋白的3篇示例参考文献(内容为示例，非真实文献)：
1. **《重组人MRPS11蛋白的制备及其在线粒体翻译中的功能研究》**
*作者：Smith J, et al.*
摘要：本研究通过原核表达系统成功制备了重组人MRPS11蛋白，并证实其参与线粒体核糖体组装，调控线粒体mRNA的翻译效率，进一步分析表明MRPS11缺失会导致氧化磷酸化功能受损。
2. **《MRPS11过表达在结直肠癌中的促癌机制及重组蛋白应用》**
*作者：Zhang Y, et al.*
摘要：利用重组MRPS11蛋白进行体外实验，发现其通过激活MAPK信号通路促进肿瘤细胞增殖，临床数据表明MRPS11在结直肠癌中高表达，可能作为潜在治疗靶点。
3. **《线粒体核糖体蛋白MRPS11的结构解析与功能重建》**
*作者：Lee H, et al.*
摘要：通过冷冻电镜技术解析了重组MRPS11蛋白的三维结构，揭示了其与16S rRNA的结合域，突变实验证实该区域对维持线粒体核糖体稳定性至关重要。
4. **《MRPS11基因突变导致代谢紊乱的重组蛋白修复研究》**
*作者：Brown K, et al.*
摘要：针对MRPS11突变患者的细胞模型，利用重组野生型MRPS11蛋白进行功能补偿实验，显著恢复了线粒体呼吸链复合体活性，为基因治疗提供新思路。
(注：以上文献为模拟生成，实际研究中请参考权威数据库如PubMed或Web of Science获取真实资料。)
**Background of Human MRPS11 Protein**
Mitochondrial ribosomal protein S11 (MRPS11) is a key component of the small subunit (28S) of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Encoded by nuclear DNA and imported into mitochondria, MRPS11 participates in assembling the ribosomal machinery responsible for translating mitochondrial DNA-encoded mRNAs. Mitochondrial ribosomes differ structurally and functionally from their cytoplasmic counterparts, reflecting adaptations to synthesize hydrophobic membrane proteins critical for oxidative phosphorylation (OXPHOS).
MRPS11 plays a role in maintaining ribosomal stability and ensuring accurate translation of mitochondrially encoded subunits of the electron transport chain complexes. Mutations in MRPS11 or dysregulation of its expression have been linked to mitochondrial disorders, often presenting with multisystem symptoms like myopathy, encephalopathy, or metabolic dysfunction due to impaired cellular energy production.
Recombinant human MRPS11 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It serves as a tool to investigate mitochondrial translation mechanisms, disease pathology, and potential therapeutic strategies. Studies also explore its interactions with other ribosomal proteins or translation factors, shedding light on ribosome biogenesis and quality control in mitochondria. Additionally, recombinant MRPS11 aids in developing biomarkers for mitochondrial dysfunction and screening compounds targeting OXPHOS deficiencies.
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