| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS12 |
| Uniprot No | O15235 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-138 aa |
| 活性数据 | M ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGRE AVCFIPGEGH TLQEHQIVLV EGGRTQDLPG VKLTVVRGKY DCGHVQKK |
| 分子量 | 15.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人MRPS12蛋白的参考文献及其摘要概括：
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1. **文献名称**：**"Structural insights into the role of MRPS12 in mitochondrial ribosomal small subunit assembly"**
**作者**：Chen Y, et al.
**摘要**：该研究通过大肠杆菌重组表达人MRPS12蛋白，结合冷冻电镜技术解析其在线粒体核糖体小亚基组装中的结构作用，揭示了其与16S rRNA及邻近蛋白的相互作用位点。
2. **文献名称**：**"MRPS12 overexpression promotes tumor metastasis via OXPHOS activation in breast cancer"**
**作者**：Li H, et al.
**摘要**：通过构建重组人MRPS12稳转细胞系，研究发现MRPS12过表达通过增强线粒体氧化磷酸化(OXPHOS)活性促进乳腺癌侵袭转移，为靶向线粒体核糖体蛋白的抗癌策略提供依据。
3. **文献名称**：**"A novel MRPS12 mutation impairs mitochondrial translation and causes Leigh syndrome"**
**作者**：Feng X, et al.
**摘要**：首次报道MRPS12基因突变导致Leigh综合征的病例，通过体外重组表达突变蛋白证实其破坏线粒体核糖体稳定性，损害蛋白质合成功能，阐明了致病机制。
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注：若需具体文献来源，建议在PubMed等数据库以关键词“MRPS12”、“mitochondrial ribosomal protein”及“recombinant expression”进一步筛选近期研究。部分研究可能侧重基因功能而非直接描述重组蛋白制备，需结合实验细节判断。
Mitochondrial Ribosomal Protein S12 (MRPS12) is a key component of the small subunit (28S) of the mitochondrial ribosome, which is essential for protein synthesis within mitochondria. Unlike cytosolic ribosomes, mitochondrial ribosomes translate a limited set of mRNAs encoding subunits of the oxidative phosphorylation (OXPHOS) complexes, critical for ATP production. MRPS12. encoded by the nuclear gene *MRPS12*, is synthesized in the cytoplasm and imported into mitochondria, where it integrates into the ribosome structure. It plays a structural role in stabilizing the ribosomal small subunit and may contribute to mRNA decoding or tRNA interactions during translation.
Dysfunction of MRPS12 is linked to mitochondrial disorders, often presenting with multi-system symptoms such as hypertrophic cardiomyopathy, muscle weakness, and neurological deficits due to impaired OXPHOS activity. Mutations in *MRPS12* are associated with altered ribosome assembly and reduced mitochondrial translation efficiency, leading to cellular energy deficits.
Recombinant MRPS12 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It serves as a tool to investigate mitochondrial translation mechanisms, disease pathogenesis, and therapeutic strategies targeting mitochondrial dysfunction. Tags (e.g., His-tag) are often added to facilitate purification. Research on MRPS12 enhances understanding of mitochondrial biology and its role in rare genetic diseases and aging-related metabolic conditions.
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