| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS17 |
| Uniprot No | Q9Y2R5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-130 aa |
| 活性数据 | KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVGK VIDPVTGKPC AGTTYLESPL SSETTQLSKN LEELNISSAQ |
| 分子量 | 14.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS17蛋白的参考文献示例(部分信息基于真实研究主题，建议通过文献数据库进一步验证)：
1. **文献名称**: *"MRPS17-mediated mitochondrial translation promotes colorectal cancer progression"*
**作者**: Li et al.
**摘要**: 研究揭示了MRPS17在结直肠癌细胞中高表达，通过重组蛋白实验证明其通过调节线粒体翻译活性促进肿瘤细胞增殖和转移。
2. **文献名称**: *"Expression and purification of recombinant human MRPS17 in E. coli for structural studies"*
**作者**: Zhang & Wang
**摘要**: 描述了利用大肠杆菌系统高效表达并纯化重组人MRPS17蛋白的方法，为其结构解析及功能研究提供了技术基础。
3. **文献名称**: *"Mitochondrial ribosomal protein MRPS17 interacts with DDX3X to regulate oxidative phosphorylation"*
**作者**: Kumar et al.
**摘要**: 通过重组蛋白互作实验发现MRPS17与DDX3X蛋白结合，共同调控线粒体氧化磷酸化过程，影响细胞能量代谢。
4. **文献名称**: *"MRPS17 deficiency impairs mitochondrial ribosome assembly leading to apoptosis in neuronal cells"*
**作者**: Chen et al.
**摘要**: 利用重组MRPS17回补实验证明，该蛋白缺失会导致线粒体核糖体组装缺陷，引发神经元凋亡，提示其在神经退行性疾病中的潜在作用。
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**注**：以上为模拟参考文献格式及内容，实际文献需通过PubMed/Google Scholar检索确认(关键词：MRPS17、mitochondrial ribosome、recombinant expression)。
**Background of Recombinant Human MRPS17 Protein**
The mitochondrial ribosomal protein S17 (MRPS17) is a key component of the mitochondrial ribosome, specifically the small subunit (28S), which is essential for protein synthesis within mitochondria. Encoded by nuclear DNA, MRPS17 is imported into mitochondria, where it participates in the assembly and stability of the ribosome. Mitochondrial ribosomes translate mitochondrial DNA (mtDNA)-encoded genes, particularly those involved in oxidative phosphorylation (OXPHOS), a critical process for cellular energy production.
MRPS17 has garnered interest due to its dual role in both mitochondrial translation and extramitochondrial functions. Studies suggest it may regulate apoptosis and interact with signaling pathways, such as p53. linking mitochondrial dysfunction to cellular stress responses. Dysregulation of MRPS17 has been implicated in diseases like cancer, neurodegenerative disorders, and metabolic syndromes, often correlating with altered mitochondrial bioenergetics.
Recombinant human MRPS17 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables researchers to study its structure, function, and interactions in vitro. This tool has been pivotal in elucidating mechanisms underlying mitochondrial disorders and exploring therapeutic strategies. Additionally, recombinant MRPS17 aids in developing assays to screen for compounds targeting mitochondrial translation or apoptosis pathways. Its study contributes to understanding the crosstalk between nuclear-encoded mitochondrial proteins and cellular health, offering insights into precision medicine for mitochondrial-related diseases.
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