| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS21 |
| Uniprot No | P82921 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-87 aa |
| 活性数据 | MAKHLKFIARTVMVQEGNVESAYRTLNRILTMDGLIEDIKHRRYYEKPCCRRQRESYERCRRIYNMEMARKINFLMRKNRADPWQGC |
| 分子量 | 37.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS21蛋白的3篇代表性文献(注：文献为虚拟示例，仅作参考)：
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1. **文献名称**: *Structural and functional analysis of MRPS21 in mitochondrial ribosome assembly*
**作者**: Müller, R. et al.
**摘要**: 本研究通过重组表达纯化人源MRPS21蛋白，结合冷冻电镜技术解析其在线粒体核糖体小亚基(SSU)中的结构定位，揭示其通过与16S rRNA结合促进核糖体组装的分子机制。
2. **文献名称**: *MRPS21 deficiency impairs mitochondrial translation and exacerbates oxidative stress in human cells*
**作者**: Chen, L. et al.
**摘要**: 作者利用CRISPR敲除细胞模型及重组MRPS21回补实验，证明该蛋白缺失导致线粒体mRNA翻译效率下降，并引发活性氧(ROS)累积，提示其在线粒体代谢中的关键作用。
3. **文献名称**: *A novel MRPS21 mutation linked to mitochondrial encephalopathy studied via recombinant protein modeling*
**作者**: Tanaka, K. et al.
**摘要**: 本研究在遗传性线粒体脑病患者中发现MRPS21点突变，通过重组突变体蛋白的功能分析，证实其导致核糖体稳定性降低，为疾病机制提供了分子层面解释。
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**备注**：实际研究中，MRPS21的相关文献多聚焦于其在线粒体翻译、疾病关联(如癌症、线粒体病)及与其他核糖体蛋白的相互作用，重组技术常作为实验工具用于蛋白功能验证。建议通过PubMed等数据库结合关键词(MRPS21. mitochondrial ribosome, recombinant expression)检索最新文献。
**Background of Recombinant Human MRPS21 Protein**
Mitochondrial ribosomal protein S21 (MRPS21) is a component of the small subunit (28S) of the mitochondrial ribosome, which is essential for mitochondrial protein synthesis. Unlike cytoplasmic ribosomes, mitochondrial ribosomes are responsible for translating a subset of mRNAs encoded by mitochondrial DNA (mtDNA), particularly those involved in oxidative phosphorylation (OXPHOS). MRPS21. encoded by nuclear DNA, is synthesized in the cytoplasm and transported to mitochondria, highlighting the coordinated regulation between nuclear and mitochondrial genomes.
Recombinant MRPS21 protein is generated through genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper post-translational modifications. Studying recombinant MRPS21 aids in understanding its structural role in ribosome assembly, as well as its functional interactions with other ribosomal proteins and mtDNA-encoded RNAs. Dysregulation of MRPS21 has been linked to mitochondrial disorders, such as impaired OXPHOS activity, which can lead to metabolic diseases, neurodegenerative conditions, and cancer. Mutations in *MRPS21* have also been associated with developmental defects, including heart and neurological abnormalities.
Furthermore, recombinant MRPS21 serves as a tool for investigating mitochondrial translation mechanisms, cellular energy metabolism, and pathologies linked to mitochondrial dysfunction. Its study may contribute to therapeutic strategies targeting mitochondrial diseases or cancer progression driven by metabolic reprogramming.
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