| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS23 |
| Uniprot No | Q9Y3D9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190 aa |
| 活性数据 | MAGSRLETVGSIFSRTRDLVRAGVLKEKPLWFDVYDAFPPLRGPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFNPNFKSTCQRFVEKYTELQKLGETDEEKLFVETGKALLAEGVILRRVGEARTQHGGSHVSRKSEHLSVRPQTALEENETQKEVPQDQHLEAPADQSKGLLPP |
| 分子量 | 46.64 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS23蛋白的模拟参考文献示例(仅供参考，实际文献需根据具体研究补充)：
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1. **文献名称**：**《重组人MRPS23蛋白的晶体结构与线粒体核糖体组装机制研究》**
**作者**：Smith A, et al.
**摘要**：本研究解析了重组表达的人MRPS23蛋白的高分辨率晶体结构，揭示了其在线粒体核糖体小亚基组装中的关键作用，并发现其C端结构域与线粒体mRNA的结合能力，为理解线粒体翻译缺陷相关疾病提供结构基础。
2. **文献名称**：**《MRPS23过表达通过调控线粒体代谢促进乳腺癌细胞增殖》**
**作者**：Chen L, et al.
**摘要**：通过重组MRPS23蛋白的功能实验，该研究发现其在乳腺癌细胞中异常高表达可通过激活线粒体OXPHOS通路促进肿瘤生长，提示其作为癌症治疗靶点的潜在价值。
3. **文献名称**：**《CRISPR/Cas9介导的MRPS23敲除诱导细胞凋亡及其机制》**
**作者**：Wang X, et al.
**摘要**：利用重组MRPS23蛋白回补实验，验证了MRPS23缺失会导致线粒体翻译功能障碍、活性氧(ROS)累积及细胞凋亡，表明其在线粒体稳态维持中的必要性。
4. **文献名称**：**《重组MRPS23蛋白在大肠杆菌中的高效表达与纯化优化》**
**作者**：Zhang Y, et al.
**摘要**：报道了一种通过密码子优化和融合标签策略在大肠杆菌中高效表达可溶性重组MRPS23蛋白的方法，为后续功能研究提供了高纯度蛋白来源。
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**备注**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索关键词“MRPS23”“mitochondrial ribosome”“recombinant protein”获取。建议结合近五年内发表的论文以确保时效性。
Recombinant human MRPS23 (Mitochondrial Ribosomal Protein S23) is a protein engineered in vitro to replicate the naturally occurring MRPS23 found in humans. As a component of the mitochondrial ribosome's small subunit (28S), MRPS23 plays a critical role in mitochondrial protein synthesis. Mitochondrial ribosomes are responsible for translating mitochondrial DNA-encoded mRNAs, particularly those essential for oxidative phosphorylation (OXPHOS) complexes, which drive cellular energy production. MRPS23 contributes to ribosome assembly, stability, and interaction with mitochondrial rRNA, ensuring efficient translation within mitochondria.
Dysregulation of MRPS23 has been implicated in mitochondrial disorders and cancer. Studies suggest its overexpression in certain tumors may enhance mitochondrial metabolism, supporting cancer cell proliferation and survival. Conversely, mutations in MRPS23 can impair OXPHOS, leading to energy deficits associated with neuromuscular or metabolic diseases. Recombinant MRPS23 is typically expressed in bacterial or mammalian systems for functional studies. It serves as a tool to investigate mitochondrial translation mechanisms, disease pathophysiology, and therapeutic targets. Its production enables structural analyses (e.g., X-ray crystallography) and interaction studies with ribosome components or potential inhibitors. Ongoing research aims to clarify its regulatory roles in mitochondrial health and disease, bridging gaps in understanding cellular bioenergetics and genetic disorders.
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