| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS27 |
| Uniprot No | Q92552 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414 aa |
| 活性数据 | MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAEY YLYKFRHSPN CWYLRNWTIH TWIRQCLKYD AQDKALYTLV NKVQYGIFPD NFTFNLLMDS FIKKENYKDA LSVVFEVMMQ EAFEVPSTQL LSLYVLFHCL AKKTDFSWEE ERNFGASLLL PGLKQKNSVG FSSQLYGYAL LGKVELQQGL RAVYHNMPLI WKPGYLDRAL QVMEKVAASP EDIKLCREAL DVLGAVLKAL TSADGASEEQ SQNDEDNQGS EKLVEQLDIE ETEQSKLPQY LERFKALHSK LQALGKIESE GLLSLTTQLV KEKLSTCEAE DIATYEQNLQ QWHLDLVQLI QREQQQREQA KQEYQAQKAA KASA |
| 分子量 | 47.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS27蛋白的3篇参考文献示例(注：文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**: *Mitochondrial Ribosomal Protein S27 is Critical for Respiratory Chain Assembly in Human Cells*
**作者**: Lee, J., et al.
**摘要**: 本研究通过重组表达人MRPS27蛋白，发现其缺失导致线粒体翻译缺陷，并影响呼吸链复合体(如复合体I和IV)的稳定性。实验验证MRPS27与线粒体18S rRNA直接结合，是核糖体小亚基组装的关键因子。
2. **文献名称**: *Heterologous Expression and Functional Analysis of MRPS27 in Yeast Mitochondria*
**作者**: Wang, Q., et al.
**摘要**: 报道在酵母线粒体中异源表达重组人MRPS27蛋白，并证明其部分挽救酵母线粒体功能缺陷。研究揭示了MRPS27在进化中的功能保守性，并提供了跨物种功能研究的模型。
3. **文献名称**: *Cryo-EM Structure of the Human Mitochondrial Ribosomal Small Subunit Reveals MRPS27’s Role in tRNA Binding*
**作者**: Patel, S., et al.
**摘要**: 通过冷冻电镜解析含重组MRPS27的人线粒体核糖体小亚基结构，发现MRPS27参与tRNA的定位和稳定，为理解线粒体翻译机制提供结构基础。
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**备注**：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台搜索“MRPS27”“mitochondrial ribosome”“recombinant protein”等关键词获取真实文献。
**Background of Recombinant Human MRPS27 Protein**
Mitochondrial ribosomal protein S27 (MRPS27) is a component of the 28S subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondrial ribosomes specialize in translating genes encoded by mitochondrial DNA (mtDNA), particularly subunits of oxidative phosphorylation (OXPHOS) complexes critical for ATP production. MRPS27. encoded by nuclear DNA, is imported into mitochondria post-translationally and plays a structural or regulatory role in ribosome assembly or translation.
Recombinant MRPS27 is produced via genetic engineering, often expressed in bacterial or mammalian systems with tags (e.g., His-tag) for purification. Its recombinant form enables studies on mitochondrial translation mechanisms, ribosome biogenesis, and interactions with other mitochondrial components. Dysregulation of MRPS27 has been implicated in mitochondrial disorders, cancer (e.g., altered metabolism in tumors), and neurodegenerative diseases linked to OXPHOS defects. Research on recombinant MRPS27 also aids in elucidating pathogenic mutations, screening therapeutic agents, and developing biomarkers for mitochondrial dysfunction. Its characterization contributes to understanding the cross-talk between nuclear and mitochondrial genomes in cellular energy homeostasis.
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