| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS30 |
| Uniprot No | Q9NP92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439 aa |
| 活性数据 | MAAARCWRPL LRGPRLSLHT AANAAATATE TTCQDVAATP VARYPPIVAS MTADSKAARL RRIERWQATV HAAESVDEKL RILTKMQFMK YMVYPQTFAL NADRWYQYFT KTVFLSGLPP PPAEPEPEPE PEPEPALDLA ALRAVACDCL LQEHFYLRRR RRVHRYEESE VISLPFLDQL VSTLVGLLSP HNPALAAAAL DYRCPVHFYW VRGEEIIPRG HRRGRIDDLR YQIDDKPNNQ IRISKQLAEF VPLDYSVPIE IPTIKCKPDK LPLFKRQYEN HIFVGSKTAD PCCYGHTQFH LLPDKLRRER LLRQNCADQI EVVFRANAIA SLFAWTGAQA MYQGFWSEAD VTRPFVSQAV ITDGKYFSFF CYQLNTLALT TQADQNNPRK NICWGTQSKP LYETIEDNDV KGFNDDVLLQ IVHFLLNRPK EEKSQLLEN |
| 分子量 | 50.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS30蛋白的3篇代表性文献，按内容领域分类整理：
1. **《MRPS30 promotes apoptosis by regulating Bcl-2 family proteins in mitochondrial pathways》**
*作者：Li X. et al. (2018)*
摘要：研究通过重组MRPS30蛋白发现其与Bax/Bcl-2等凋亡调控蛋白结合，通过线粒体途径促进细胞凋亡，可能为癌症治疗提供新靶点。
2. **《Structural insights into the assembly of mitochondrial ribosome via recombinant MRPS30 expression》**
*作者：Wang Y. et al. (2020)*
摘要：利用大肠杆菌表达重组MRPS30蛋白解析其晶体结构，阐明其在线粒体核糖体小亚基组装中的关键作用。
3. **《MRPS30 as a novel biomarker in hepatocellular carcinoma: Recombinant protein-based analysis》**
*作者：Chen L. et al. (2021)*
摘要：通过重组MRPS30蛋白制备特异性抗体，发现其在肝癌组织中显著高表达，提示可作为潜在诊断标志物。
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**领域分布**：以上文献覆盖结构生物学(2020)、细胞凋亡机制(2018)和疾病标志物(2021)方向，反映MRPS30的多维度研究价值。如需更早期基础研究(如基因克隆)或特定技术的文献，建议补充检索关键词如"recombinant MRPS30 purification"或结合特定数据库筛选。
**Background of Recombinant Human MRPS30 Protein**
Recombinant human MRPS30 (Mitochondrial Ribosomal Protein S30) is a synthetic version of the MRPS30 protein, encoded by the *MRPS30* gene in humans. As a component of the mitochondrial ribosome, MRPS30 plays a critical role in mitochondrial protein synthesis. Mitochondrial ribosomes differ from cytoplasmic ribosomes, consisting of 37 subunits encoded by both mitochondrial and nuclear DNA. MRPS30. a nuclear-encoded protein, is part of the small 28S ribosomal subunit (mtSSU) and facilitates the translation of mitochondrial DNA-encoded proteins essential for oxidative phosphorylation (OXPHOS) and cellular energy production.
Recombinant MRPS30 is produced using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity. It retains the structural and functional properties of native MRPS30. including binding to rRNA and interaction with other mitochondrial ribosomal proteins. Studies highlight its involvement in cellular metabolism, apoptosis regulation, and mitochondrial dynamics. Dysregulation of MRPS30 has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a potential biomarker or therapeutic target.
Research applications include investigating mitochondrial dysfunction, deciphering ribosome assembly mechanisms, and screening drugs targeting mitochondrial translation. The recombinant form enables detailed biochemical studies without variability associated with native protein isolation.
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