| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS31 |
| Uniprot No | Q92665 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 66-395 aa |
| 活性数据 | ICSKK DKQSVRTEET SKETSESQDS EKENTKKDLL GIIKGMKVEL STVNVRTTKP PKRRPLKSLE ATLGRLRRAT EYAPKKRIEP LSPELVAAAS AVADSLPFDK QTTKSELLSQ LQQHEEESRA QRDAKRPKIS FSNIISDMKV ARSATARVRS RPELRIQFDE GYDNYPGQEK TDDLKKRKNI FTGKRLNIFD MMAVTKEAPE TDTSPSLWDV EFAKQLATVN EQPLQNGFEE LIQWTKEGKL WEFPINNEAG FDDDGSEFHE HIFLEKHLES FPKQGPIRHF MELVTCGLSK NPYLSVKQKV EHIEWFRNYF NEKKDILKES NIQFN |
| 分子量 | 45.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS31蛋白的3篇示例参考文献(注：文献为虚构示例，仅作格式参考)：
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1. **标题**：*Recombinant Human MRPS31 Expression and Its Role in Mitochondrial Translation*
**作者**：Smith A, Chen B.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化重组人MRPS31蛋白，发现其与线粒体16S rRNA结合，对维持线粒体核糖体小亚基的组装和蛋白质翻译效率至关重要。
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2. **标题**：*Structural Insights into MRPS31 Using Cryo-EM: Implications for Mitochondrial Dysfunction*
**作者**：Wang X, Li Y.
**摘要**：通过冷冻电镜解析重组MRPS31的复合物结构，揭示了其与线粒体核糖体其他亚基的相互作用界面，突变实验表明其关键结构域缺陷会导致呼吸链酶活性下降。
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3. **标题**：*MRPS31 Overexpression Enhances Apoptosis Resistance in Cancer Cells*
**作者**：Kim H, Patel R.
**摘要**：重组MRPS31在HeLa细胞中的过表达研究发现，该蛋白通过抑制线粒体膜通透性转换孔(mPTP)的开放，降低细胞色素C释放，从而抑制凋亡通路。
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(注：实际文献需通过数据库如PubMed或Google Scholar检索确认。)
The mitochondrial ribosomal protein S31 (MRPS31) is a key component of the mitochondrial ribosome, specifically the 39S subunit of the large mitoribosomal complex. Encoded by nuclear DNA, this protein is synthesized in the cytoplasm and imported into mitochondria, where it plays a critical role in mitochondrial protein synthesis. Mitochondrial ribosomes are responsible for translating mitochondrial DNA-encoded mRNAs, primarily those involved in oxidative phosphorylation complexes essential for ATP production. MRPS31. along with other ribosomal proteins, ensures proper assembly and structural integrity of the ribosome, facilitating accurate translation. Dysregulation of MRPS31 has been linked to mitochondrial dysfunction, implicated in various diseases such as neurodegenerative disorders, cancer, and metabolic syndromes. Research on recombinant human MRPS31 (produced via heterologous expression systems like *E. coli* or mammalian cells) enables biochemical and functional studies, aiding in deciphering its molecular interactions and role in disease mechanisms. Recombinant forms are also vital for antibody production, structural studies (e.g., cryo-EM), and drug discovery targeting mitochondrial translation defects. Its study provides insights into mitochondrial biology and potential therapeutic strategies for disorders tied to energy metabolism anomalies.
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