纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MRPS33 |
Uniprot No | Q9Y291 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-106 aa |
活性数据 | MSSLSEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLRGKEKPKKGEGKRAAKRK |
分子量 | 39 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS33蛋白的3篇参考文献示例(请注意,文献内容为示例性质,实际引用需根据真实论文补充):
1. **文献名称**:*Mitochondrial ribosomal protein S33 regulates cell apoptosis via the JNK pathway in colorectal cancer*
**作者**:Zhang Y, Li H, Wang X, et al.
**摘要概括**:该研究探讨了MRPS33在结直肠癌细胞线粒体翻译调控中的作用,发现其表达异常通过激活JNK信号通路促进细胞凋亡,研究利用重组MRPS33蛋白验证了其对线粒体功能的影响。
2. **文献名称**:*Cloning and characterization of human mitochondrial ribosomal protein S33 (MRPS33) reveals its role in oxidative phosphorylation*
**作者**:Chen L, Liu Q, Sun Y.
**摘要概括**:作者通过克隆和表达重组人MRPS33蛋白,分析了其在氧化磷酸化复合物组装中的功能,实验表明MRPS33缺失会导致线粒体呼吸链活性显著下降。
3. **文献名称**:*MRPS33 mutations disrupt protein synthesis machinery and cause developmental defects in zebrafish*
**作者**:Wang T, Chen Z, Xu J, et al.
**摘要概括**:本研究利用重组MRPS33蛋白在斑马鱼模型中恢复突变表型,证明了MRPS33在线粒体蛋白质合成中的关键作用及其对胚胎发育的影响。
**备注**:若需真实文献,建议通过PubMed、Google Scholar等数据库以关键词“MRPS33”、“recombinant human MRPS33”或“mitochondrial ribosome”检索,并筛选涉及蛋白重组表达或功能机制的研究。
Mitochondrial ribosomal protein S33 (MRPS33) is a key component of the small subunit (28S) of the mitochondrial ribosome, which is essential for synthesizing proteins encoded by mitochondrial DNA (mtDNA). As part of the machinery responsible for mitochondrial translation, MRPS33 plays a critical role in maintaining oxidative phosphorylation (OXPHOS) and cellular energy production. Human MRPS33 is encoded by the nuclear gene *MRPS33*, reflecting the co-evolutionary dependence between nuclear and mitochondrial genomes. Dysfunction in mitochondrial ribosomal proteins, including MRPS33. is linked to mitochondrial disorders characterized by impaired energy metabolism, neurodegeneration, or metabolic syndromes. Recombinant human MRPS33 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, is widely used to study its structural and functional roles, interactions within the ribosome, and implications in disease mechanisms. Such recombinant tools enable investigations into how MRPS33 mutations disrupt mitochondrial translation, contribute to pathological stress responses, or affect apoptosis. Additionally, recombinant MRPS33 serves as a reagent for developing diagnostics or therapies targeting mitochondrial dysfunction. Ongoing research aims to elucidate its post-translational modifications, tissue-specific expression patterns, and potential as a biomarker in mitochondrial-related pathologies.
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