| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS5 |
| Uniprot No | P82675 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430 aa |
| 活性数据 | MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSFF TKLTADELWK GALAETGAGA KKGRGKRTKK KKRKDLNRGQ IIGEGRYGFL WPGLNVPLMK NGAVQTIAQR SKEEQEKVEA DMIQQREEWD RKKKMKVKRE RGWSGNSWGG ISLGPPDPGP CGETYEDFDT RILEVRNVFT MTAKEGRKKS IRVLVAVGNG KGAAGFSIGK ATDRMDAFRK AKNRAVHHLH YIERYEDHTI FHDISLRFKR THIKMKKQPK GYGLRCHRAI ITICRLIGIK DMYAKVSGSI NMLSLTQGLF RGLSRQETHQ QLADKKGLHV VEIREECGPL PIVVASPRGP LRKDPEPEDE VPDVKLDWED VKTAQGMKRS VWSNLKRAAT |
| 分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS5蛋白的参考文献示例(部分为假设性概括，若需真实文献请进一步验证)：
1. **文献名称**：*Structural and functional characterization of human mitochondrial ribosomal protein S5 (MRPS5)*
**作者**：Zhang Y et al.
**摘要**：本研究利用大肠杆菌表达系统成功纯化了重组人MRPS5蛋白，并通过质谱和晶体学解析了其三维结构。实验发现，MRPS5在维持线粒体核糖体小亚基稳定性中起关键作用，并通过与mRNA相互作用参与翻译起始。
2. **文献名称**：*MRPS5 mutations impair mitochondrial translation in human cell lines*
**作者**：Wang L et al.
**摘要**：通过构建重组MRPS5蛋白的突变体，研究发现其缺失会导致线粒体蛋白合成效率下降，引发呼吸链复合物组装缺陷，并与罕见代谢疾病的发病机制相关。
3. **文献名称**：*Recombinant MRPS5 as a potential biomarker for colorectal cancer progression*
**作者**：Kim S et al.
**摘要**：该研究在昆虫细胞表达系统中获得高纯度重组MRPS5蛋白，并发现其在结直肠癌组织中异常高表达，可能通过调控线粒体氧化磷酸化促进肿瘤细胞增殖。
4. **文献名称**：*Expression and purification of human MRPS5 for antibody generation*
**作者**：Chen X et al.
**摘要**：报道了一种优化的哺乳动物表达体系制备重组MRPS5蛋白的方法，利用该蛋白制备的多克隆抗体可用于免疫印迹和免疫组化，为线粒体功能障碍相关疾病提供研究工具。
**注**：以上文献信息为示例性质，实际研究需通过PubMed、Google Scholar等平台检索关键词“recombinant MRPS5”或“human MRPS5 protein”获取具体资料。
Recombinant Human Mitochondrial Ribosomal Protein S5 (MRPS5) is a key component of the mitochondrial ribosome, specifically the 28S subunit of the mitochondrial small ribosomal subunit (mt-SSU). Encoded by the nuclear gene MRPS5. this protein is synthesized in the cytoplasm and transported to mitochondria, where it facilitates the assembly and stability of the ribosome structure. Mitochondrial ribosomes are essential for translating mitochondrial DNA-encoded mRNAs, particularly those involved in oxidative phosphorylation (OXPHOS) complexes, which are critical for cellular energy production. MRPS5 plays a role in maintaining translation fidelity and interacts with other ribosomal proteins and mitochondrial RNA.
Recombinant MRPS5 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional and structural studies. Its study is relevant to mitochondrial disorders, neurodegenerative diseases, and cancer, as mitochondrial dysfunction is linked to these conditions. Research applications include investigating ribosome biogenesis, protein synthesis mechanisms, and pathways implicated in cellular metabolism. The recombinant form allows detailed biochemical analysis, aiding drug discovery and diagnostics for mitochondria-related pathologies.
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