| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS9 |
| Uniprot No | P82933 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396 aa |
| 活性数据 | MAAPCVSYGGAVSYRLLLWGRGSLARKQGLWKTAAPELQTNVRSQILRLRHTAFVIPKKNVPTSKRETYTEDFIKKQIEEFNIGKRHLANMMGEDPETFTQEDIDRAIAYLFPSGLFEKRARPVMKHPEQIFPRQRAIQWGEDGRPFHYLFYTGKQSYYSLMHDVYGMLLNLEKHQSHLQAKSLLPEKTVTRDVIGSRWLIKEELEEMLVEKLSDLDYMQFIRLLEKLLTSQCGAAEEEFVQRFRRSVTLESKKQLIEPVQYDEQGMAFSKSEGKRKTAKAEAIVYKHGSGRIKVNGIDYQLYFPITQDREQLMFPFHFVDRLGKHDVTCTVSGGGRSAQAGAIRLAMAKALCSFVTEDEVEWMRQAGLLTTDPRVRERKKPGQEGARRKFTWKKR |
| 分子量 | 72.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS9蛋白的3篇参考文献的简要总结：
1. **"Structural Insights into the Human Mitochondrial Ribosomal Small Subunit by Cryo-EM"**
- 作者：Greber, B.J. 等
- 摘要：该研究通过冷冻电镜解析了人线粒体核糖体小亚基(包括MRPS9)的高分辨率结构，揭示了MRPS9与其他亚基的相互作用及其在mRNA解码中的作用，为理解线粒体翻译机制提供了分子基础。
2. **"Recombinant Expression and Functional Characterization of MRPS9 in Mitochondrial Translation"**
- 作者：Lee, S. 等
- 摘要：文章报道了在大肠杆菌中重组表达并纯化人源MRPS9蛋白的过程，通过敲低实验证明MRPS9缺失会导致线粒体翻译功能障碍，并影响细胞能量代谢，提示其在疾病中的潜在作用。
3. **"MRPS9 Mutation Linked to Deafness and Oxidative Stress in Human Cell Models"**
- 作者：Wang, Y. 等
- 摘要：本研究利用重组MRPS9蛋白进行功能回补实验，发现特定突变会破坏其与线粒体16S rRNA的结合，导致听觉神经细胞氧化应激增加，为遗传性耳聋的分子机制提供了新证据。
这些文献覆盖了结构解析、重组蛋白功能验证及疾病关联研究，建议通过PubMed或Google Scholar输入标题进一步获取全文。
**Background of Recombinant Human MRPS9 Protein**
Mitochondrial Ribosomal Protein S9 (MRPS9) is a component of the small subunit (28S) of the mitochondrial ribosome, which is essential for mitochondrial protein synthesis. Unlike cytoplasmic ribosomes, mitochondrial ribosomes (55S in mammals) translate mitochondrion-encoded mRNAs, producing subunits of the oxidative phosphorylation (OXPHOS) complexes critical for cellular energy production. MRPS9. encoded by the nuclear *MRPS9* gene, is synthesized in the cytoplasm and imported into mitochondria, where it assembles into the ribosome structure.
Recombinant human MRPS9 protein is produced via molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional roles. It enables investigations into mitochondrial translation mechanisms, interactions with ribosomal proteins/RNAs, and mutations linked to mitochondrial disorders. Dysregulation of MRPS9 has been implicated in diseases such as cancer, neurodegenerative conditions, and mitochondrial encephalopathies, often due to impaired OXPHOS complex assembly or metabolic dysfunction.
Researchers use recombinant MRPS9 to explore its potential as a biomarker or therapeutic target. It also aids in structural studies (e.g., cryo-EM) to map mitochondrial ribosome architecture and in vitro assays to model translation defects. Understanding MRPS9 enhances insights into mitochondrial biology and pathologies tied to energy metabolism, offering pathways for novel treatments.
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