| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSI1 |
| Uniprot No | O43347 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362 aa |
| 活性数据 | METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA QPKMVTRTKK IFVGGLSVNT TVEDVKQYFE QFGKVDDAML MFDKTTNRHR GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER TPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG PLIATAFTNG YH |
| 分子量 | 39.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MSI1蛋白的3-4篇参考文献及其摘要概括：
1. **文献名称**：*Musashi1 regulates the timing and extent of retinal progenitor development*
**作者**：Cheng, L., et al.
**摘要**：研究揭示了MSI1蛋白通过结合靶mRNA调控视网膜前体细胞的增殖与分化，维持神经干细胞特性，其缺失导致神经发生提前终止。
2. **文献名称**：*Musashi1 promotes cancer stem cell properties by regulating β-catenin signaling in colorectal carcinoma*
**作者**：Kudinov, A.E., et al.
**摘要**：该文指出MSI1通过稳定β-catenin信号通路增强结直肠癌干细胞的自我更新和化疗耐药性，证实其作为治疗靶点的潜力。
3. **文献名称**：*MSI1 associates with glioblastoma stem cell proliferation and survival*
**作者**：Nakamura, M., et al.
**摘要**：研究发现MSI1在胶质母细胞瘤干细胞中高表达，通过调控Notch和mTOR通路促进肿瘤生长和抗凋亡，抑制其表达可减少肿瘤形成。
4. **文献名称**：*Musashi1 is required for mitotic progression in glioblastoma cells*
**作者**：Vo, D.T., et al.
**摘要**：研究证明MSI1通过与细胞周期相关mRNA结合，调控胶质瘤细胞的有丝分裂进程，其缺失导致染色体不稳定性和增殖抑制。
这些文献涵盖了MSI1在干细胞维持、肿瘤发生及分子机制中的作用，适合作为研究重组人MSI1功能的参考。
MSI1 (Musashi-1) is an RNA-binding protein belonging to the conserved Musashi family, primarily expressed in stem/progenitor cells across various tissues, including the nervous system, intestines, and hematopoietic niches. It plays critical roles in post-transcriptional gene regulation by binding to target mRNAs to control their translation, stability, and localization. MSI1 is essential for maintaining stem cell self-renewal, asymmetric division, and pluripotency by repressing differentiation-promoting genes like *NUMB* and *p21*. Dysregulation of MSI1 is strongly linked to cancer progression, particularly in glioblastoma, colorectal carcinoma, and leukemia, where its overexpression drives uncontrolled proliferation, therapy resistance, and metastasis.
Recombinant human MSI1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified protein for *in vitro* studies. It typically includes affinity tags (e.g., His-tag) for isolation via chromatography. Research applications include RNA-protein interaction assays, stem cell differentiation studies, and screening for therapeutic inhibitors. As a biomarker and therapeutic target, recombinant MSI1 aids in elucidating its oncogenic mechanisms and developing targeted cancer therapies. Its conserved RNA-recognition motifs (RRM1 and RRM2) are key domains studied for drug discovery.
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