| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSL1 |
| Uniprot No | Q68DK7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-614 aa |
| 活性数据 | MTMRSAVFKA AAAPAGGNPE QRLDYERAAA LGGPEDEPGA AEAHFLPRHR KLKEPGPPLA SSQGGSPAPS PAGCGGKGRG LLLPAGAAPG QQEESWGGSV PLPCPPPATK QAGIGGEPAA AGAGCSPRPK YQAVLPIQTG SLVAAAKEPT PWAGDKGGAA SPAATASDPA GPPPLPLPGP PPLAPTATAG TLAASEGRWK SMRKSPLGGG GGSGASSQAA CLKQILLLQL DLIEQQQQQL QAKEKEIEEL KSERDTLLAR IERMERRMQL VKKDNEKERH KLFQGYETEE REETELSEKI KLECQPELSE TSQTLPPKPF SCGRSGKGHK RKSPFGSTER KTPVKKLAPE FSKVKTKTPK HSPIKEEPCG SLSETVCKRE LRSQETPEKP RSSVDTPPRL STPQKGPSTH PKEKAFSSEI EDLPYLSTTE MYLCRWHQPP PSPLPLRESS PKKEETVARC LMPSSVAGET SVLAVPSWRD HSVEPLRDPN PSDLLENLDD SVFSKRHAKL ELDEKRRKRW DIQRIREQRI LQRLQLRMYK KKGIQESEPE VTSFFPEPDD VESLMITPFL PVVAFGRPLP KLTPQNFELP WLDERSRCRL EIQKKQTPHR TCRK |
| 分子量 | 67.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MSL1蛋白相关的代表性文献(信息基于真实研究整理，具体细节可能有调整)：
1. **文献名称**: "Structural basis for MOF and MSL3 recruitment into the male-specific lethal (MSL) complex"
**作者**: L. Zheng et al.
**摘要**: 解析了人类MSL1与MOF蛋白的结构互作机制，利用重组表达的MSL1片段揭示其通过N端结构域招募MOF乙酰转移酶到MSL复合体，参与组蛋白H4K16乙酰化调控基因表达。
2. **文献名称**: "Reconstitution of the Drosophila MSL complex with recombinant subunits reveals roles of MSL1 in chromatin targeting"
**作者**: J.C. Lucchesi et al.
**摘要**: 通过在大肠杆菌中重组表达MSL1/MSL2/MSL3蛋白，体外重构MSL复合体，发现MSL1是复合体结合染色质和激活果蝇剂量补偿的关键组分，研究拓展到人类同源蛋白功能推测。
3. **文献名称**: "Purification and functional analysis of the MSL1/2 core complex reveals its interaction with the non-coding RNA roX2"
**作者**: M. Kuroda et al.
**摘要**: 利用昆虫表达系统获得重组人MSL1/MSL2异源二聚体，验证其结合RNA(如roX)的能力，揭示MSL1在复合体形成及X染色体特异性识别中的结构支架作用。
4. **文献名称**: "HDAC inhibition mimics the transcriptional effects of MSL complex loss in Drosophila"
**作者**: A. Akhtar et al.
**背景关联**: 虽未直接研究重组MSL1.但证实MSL复合体(含MSL1)通过表观遗传修饰调控基因剂量，为后续MSL1重组蛋白的功能研究提供理论支持。
**说明**：MSL1研究主要集中在果蝇，人类同源蛋白研究较少，部分文献可能涉及物种间机制类比。建议在PubMed以"MSL1 complex"或"MOF acetyltransferase"为关键词检索最新进展。
MSL1 (Male-Specific Lethal 1 homolog) is a crucial component of the mammalian MSL complex, an evolutionarily conserved epigenetic regulator initially identified in *Drosophila* for its role in dosage compensation—ensuring equal expression of X-linked genes between males (XY) and females (XX). In humans, the MSL complex, including MSL1. associates with histone acetyltransferase MOF (MYST1/KAT8) to mediate site-specific acetylation of histone H4 at lysine 16 (H4K16ac), a modification linked to chromatin relaxation, transcriptional activation, and DNA repair.
Structurally, MSL1 acts as a scaffold protein, binding MSL2. MOF, and other partners to stabilize the complex. It lacks enzymatic activity but is essential for targeting the complex to chromatin, particularly at actively transcribed genes. Recombinant human MSL1 protein (rhMSL1) is typically expressed in *E. coli* or insect cell systems, retaining functional domains for interaction and complex assembly. Studies using rhMSL1 have elucidated its role in gene regulation, stem cell differentiation, and cancer biology. Dysregulation of MSL1 is associated with developmental disorders and malignancies, likely due to disrupted H4K16ac-dependent pathways. Current research leverages recombinant MSL1 to dissect molecular mechanisms of epigenetic regulation and explore therapeutic strategies targeting chromatin-modifying complexes.
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