| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSL2L1 |
| Uniprot No | Q9HCI7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-577 aa |
| 活性数据 | MNPVNATALY ISASRLVLNY DPGDPKAFTE INRLLPYFRQ SLSCCVCGHL LQDPIAPTNS TCQHYVCKTC KGKKMMMKPS CSWCKDYEQF EENKQLSILV NCYKKLCEYI TQTTLARDII EAVDCSSDIL ALLNDGSLFC EETEKPSDSS FTLCLTHSPL PSTSEPTTDP QASLSPMSES TLSIAIGSSV INGLPTYNGL SIDRFGINIP SPEHSNTIDV CNTVDIKTED LSDSLPPVCD TVATDLCSTG IDICSFSEDI KPGDSLLLSV EEVLRSLETV SNTEVCCPNL QPNLEATVSN GPFLQLSSQS LSHNVFMSTS PALHGLSCTA ATPKIAKLNR KRSRSESDSE KVQPLPISTI IRGPTLGASA PVTVKRESKI SLQPIATVPN GGTTPKISKT VLLSTKSMKK SHEHGSKKSH SKTKPGILKK DKAVKEKIPS HHFMPGSPTK TVYKKPQEKK GCKCGRATQN PSVLTCRGQR CPCYSNRKAC LDCICRGCQN SYMANGEKKL EAFAVPEKAL EQTRLTLGIN VTSIAVRNAS TSTSVINVTG SPVTTFLAAS THDDKSLDEA IDMRFDC |
| 分子量 | 62.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MSL2L1蛋白的3篇参考文献示例，基于相关领域研究的合理推测构建：
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1. **文献名称**：*Structural and functional analysis of recombinant human MSL2L1 in the MOF histone acetyltransferase complex*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人MSL2L1蛋白，发现其与组蛋白乙酰转移酶MOF直接相互作用，并证明MSL2L1对复合体在染色质靶向和乙酰化活性中起关键作用。
2. **文献名称**：*Optimization of recombinant MSL2L1 expression in insect cells and characterization of its chromatin-binding properties*
**作者**：Zhang Y, et al.
**摘要**：通过昆虫细胞表达系统高效生产重组人MSL2L1蛋白，纯化后结合体外染色质沉淀实验，揭示其对X染色体特定区域的高亲和力，暗示其在剂量补偿中的潜在功能。
3. **文献名称**：*MSL2L1 interacts with non-coding RNA to regulate gene expression via recombinant protein-mediated assays*
**作者**：Lee J, et al.
**摘要**：利用重组表达的MSL2L1蛋白进行RNA免疫共沉淀(RIP)实验，发现其与特定长链非编码RNA结合，共同调控靶基因的转录激活，为剂量补偿机制提供新视角。
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**注**：上述文献为示例性构建，若需真实引用，请通过学术数据库(如PubMed、Google Scholar)以“MSL2L1”“重组表达”或“MOF复合体”等关键词检索核实。MSL2L1相关研究可能较少，实际文献可能集中于其同源蛋白或复合体功能研究。
MSL2L1 (Male-Specific Lethal 2-Like 1), also known as MSL2. is a key component of the evolutionarily conserved Male-Specific Lethal (MSL) complex. In Drosophila, the MSL complex mediates dosage compensation by upregulating transcription of the single male X chromosome through histone H4 lysine 16 acetylation (H4K16ac). In humans, the homolog MSL2L1 retains functional conservation, participating in chromatin modification and transcriptional regulation. It interacts with histone acetyltransferase MOF (KAT8) to facilitate site-specific acetylation, influencing chromatin structure and gene expression.
Structurally, MSL2L1 contains a chromodomain for chromatin interaction and a RING finger domain implicated in ubiquitin ligase activity. Studies suggest its involvement in DNA repair, cell cycle regulation, and stem cell maintenance. Dysregulation of MSL2L1 has been linked to cancers, where overexpression correlates with poor prognosis, potentially by promoting oncogene expression or genomic instability. Recombinant MSL2L1 protein is widely used in vitro to study its biochemical interactions, enzymatic roles, and mechanisms in epigenetic regulation. Its engineered variants also aid in exploring therapeutic targets related to chromatin remodeling pathways. Despite progress, precise context-dependent functions and regulatory networks of MSL2L1 in human diseases remain under active investigation.
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