关键信息
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基因名
KRT10
- 应用
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别名
KRT10；KPP；Keratin. type I cytoskeletal 10
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P13645
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表达区间
1-584aa
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氨基酸序列
MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY
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分子量
62.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KRT10, or keratin 10, is a member of the keratin family of proteins, primarily expressed in the epithelial cells of the skin. It plays a crucial role in maintaining epidermal integrity and homeostasis. Mutations or deficiencies in KRT10 have been implicated in various skin disorders, including epidermolytic hyperkeratosis and palmoplantar keratoderma, which are characterized by abnormal keratinocyte differentiation and disrupted skin barrier function. The study of KRT10 recombinant protein holds significant potential for understanding the molecular mechanisms underlying these conditions and for developing targeted therapeutic strategies. By producing KRT10 in recombinant systems, researchers can investigate its structural properties, interactions with other cytoskeletal proteins, and regulatory pathways governing keratinocyte function. Moreover, the recombinant protein serves as a valuable tool for studying skin biology and developing biomaterials for regenerative medicine or skin repair. Research into KRT10 also provides insights into the evolutionary adaptations of keratin proteins in various species, enhancing our understanding of skin biology across species. Overall, the investigation of KRT10 recombinant protein is pivotal not only for advancing dermatological research but also for identifying novel approaches to treat keratin-related diseases.
