| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSX1 |
| Uniprot No | P28360 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303 aa |
| 活性数据 | MAPAADMTSL PLGVKVEDSA FGKPAGGGAG QAPSAAAATA AAMGADEEGA KPKVSPSLLP FSVEALMADH RKPGAKESAL APSEGVQAAG GSAQPLGVPP GSLGAPDAPS SPRPLGHFSV GGLLKLPEDA LVKAESPEKP ERTPWMQSPR FSPPPARRLS PPACTLRKHK TNRKPRTPFT TAQLLALERK FRQKQYLSIA ERAEFSSSLS LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPPAA FGLSFPLGGP AAVAAAAGAS LYGASGPFQR AALPVAPVGL YTAHVGYSMY HLT |
| 分子量 | 31.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人MSX1蛋白的参考文献及其简要内容概括：
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1. **文献名称**: *"MSX1 suppresses osteoblast differentiation by regulating β-catenin nuclear translocation in craniofacial development"*
**作者**: Han et al.
**摘要**: 研究利用重组人MSX1蛋白，发现其通过抑制β-catenin的核转位，负向调控成骨细胞分化，影响颅面骨骼发育。实验证明MSX1重组蛋白可阻断Wnt信号通路。
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2. **文献名称**: *"Recombinant MSX1 protein expression in E. coli and its functional analysis in dental mesenchymal stem cells"*
**作者**: Bendall et al.
**摘要**: 报道在大肠杆菌中高效表达并纯化重组人MSX1蛋白，证明其通过结合靶基因启动子抑制牙源间充质干细胞的成牙分化，揭示了其在牙齿发育中的转录抑制机制。
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3. **文献名称**: *"MSX1 interacts with p53 to regulate cellular senescence in head and neck squamous cell carcinoma"*
**作者**: Simeone et al.
**摘要**: 通过重组MSX1蛋白实验，揭示其与p53蛋白相互作用，诱导头颈鳞癌细胞衰老，并证实其通过调控p21等衰老相关基因抑制肿瘤进展。
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4. **文献名称**: *"Structural and functional characterization of the homeodomain of recombinant human MSX1 protein"*
**作者**: Lee & Kim
**摘要**: 解析重组人MSX1同源结构域的晶体结构，结合体外DNA结合实验，阐明其特异性识别TAAT序列的分子机制，为理解MSX1在胚胎发育中的功能提供结构基础。
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*注：以上文献信息为模拟示例，实际引用需以具体发表文章为准。建议通过PubMed或Web of Science以“recombinant human MSX1 protein”为关键词检索最新研究。*
**Background of Recombinant Human MSX1 Protein**
MSX1 (Msh Homeobox 1) is a member of the muscle segment homeobox (MSH) gene family, encoding a transcription factor critical during embryonic development. It plays pivotal roles in organogenesis, cell differentiation, and pattern formation, particularly in craniofacial, limb, nervous system, and tooth development. MSX1 regulates gene expression by binding to DNA through its conserved homeodomain, often interacting with signaling pathways like BMP, FGF, and Wnt to control cell fate decisions, epithelial-mesenchymal interactions, and tissue morphogenesis.
Recombinant human MSX1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. This enables functional studies on its role in stem cell maintenance, differentiation inhibition, and tissue regeneration. Researchers utilize recombinant MSX1 to explore its therapeutic potential, such as in dental regeneration or craniofacial defect repair. Additionally, mutations in *MSX1* are linked to congenital disorders like tooth agenesis and cleft lip/palate, making the recombinant protein a tool for mechanistic and drug discovery studies.
Its applications extend to developmental biology, regenerative medicine, and disease modeling, underscoring its importance in both basic research and translational medicine.
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