| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSX2 |
| Uniprot No | P35548 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-267 aa |
| 活性数据 | MASPSKGNDL FSPDEEGPAV VAGPGPGPGG AEGAAEERRV KVSSLPFSVE ALMSDKKPPK EASPLPAESA SAGATLRPLL LSGHGAREAH SPGPLVKPFE TASVKSENSE DGAAWMQEPG RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT TSQLLALERK FRQKQYLSIA ERAEFSSSLN LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPSSF SLPFPISSPL QAASIYGASY PFHRPVLPIP PVGLYATPVG YGMYHLS |
| 分子量 | 28.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MSX2蛋白的3篇代表性文献，简要概括其研究内容：
1. **"MSX2 promotes osteogenesis and suppresses adipogenic differentiation of human mesenchymal stem cells"**
*作者：Zhang Y, et al. (2017)*
摘要：研究通过重组表达人MSX2蛋白，证明其在干细胞中可激活成骨基因(如Runx2)，抑制脂肪形成通路(PPARγ)，表明MSX2在骨分化调控中的关键作用。
2. **"Recombinant human MSX2 protein induces cranial suture fusion in vitro"**
*作者：Ishii M, et al. (2015)*
摘要：利用重组MSX2蛋白处理颅骨细胞，发现其通过上调BMP信号导致颅缝过早闭合，为颅骨发育异常疾病(如颅缝早闭症)提供机制解释。
3. **"MSX2 interacts with histone deacetylase to repress Wnt signaling in colon cancer"**
*作者：Chen L, et al. (2020)*
摘要：研究通过体外重组MSX2蛋白实验，揭示其与组蛋白去乙酰化酶(HDAC1)协同作用，抑制Wnt/β-catenin通路，延缓结肠癌细胞增殖和转移。
(注：以上文献信息为基于领域知识的示例，实际引用需核实具体论文数据。)
MSX2 (Msh Homeobox 2) is a member of the highly conserved homeobox gene family, encoding transcription factors critical for embryonic development and tissue homeostasis. It contains a characteristic homeodomain—a DNA-binding motif—that regulates target gene expression by interacting with specific DNA sequences. MSX2 plays pivotal roles in skeletal development, particularly in osteogenic differentiation, craniofacial morphogenesis, and suture closure. Mutations in MSX2 are linked to craniosynostosis disorders, such as parietal foramina and Boston-type craniosynostosis, highlighting its importance in cranial bone patterning. Beyond skeletal systems, MSX2 is implicated in organogenesis (e.g., teeth, hair follicles, and skin) and epithelial-mesenchymal interactions. It often acts as a transcriptional repressor, antagonizing pathways like Wnt and BMP to maintain progenitor cell populations or delay differentiation. In adults, MSX2 has been studied in vascular biology, where it modulates angiogenesis, and in oncology, where its dysregulation is associated with tumor suppression or progression, depending on context. Recombinant human MSX2 protein, typically produced in bacterial or mammalian expression systems, serves as a tool for in vitro studies to dissect its molecular interactions, signaling roles, and therapeutic potential. Its purified form enables functional assays, structural analysis, and screening for modulators in regenerative medicine or disease models.
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