| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTMR1 |
| Uniprot No | Q13613 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665 aa |
| 活性数据 | MDRPAAAAAA GCEGGGGPNP GPAGGRRPPR AAGGATAGSR QPSVETLDSP TGSHVEWCKQ LIAATISSQI SGSVTSENVS RDYKALRDGN KLAQMEEAPL FPGESIKAIV KDVMYICPFM GAVSGTLTVT DFKLYFKNVE RDPHFILDVP LGVISRVEKI GAQSHGDNSC GIEIVCKDMR NLRLAYKQEE QSKLGIFENL NKHAFPLSNG QALFAFSYKE KFPINGWKVY DPVSEYKRQG LPNESWKISK INSNYEFCDT YPAIIVVPTS VKDDDLSKVA AFRAKGRVPV LSWIHPESQA TITRCSQPLV GPNDKRCKED EKYLQTIMDA NAQSHKLIIF DARQNSVADT NKTKGGGYES ESAYPNAELV FLEIHNIHVM RESLRKLKEI VYPSIDEARW LSNVDGTHWL EYIRMLLAGA VRIADKIESG KTSVVVHCSD GWDRTAQLTS LAMLMLDSYY RTIKGFETLV EKEWISFGHR FALRVGHGND NHADADRSPI FLQFVDCVWQ MTRQFPSAFE FNELFLITIL DHLYSCLFGT FLCNCEQQRF KEDVYTKTIS LWSYINSQLD EFSNPFFVNY ENHVLYPVAS LSHLELWVNY YVRWNPRMRP QMPIHQNLKE LLAVRAELQK RVEGLQREVA TRAVSSSSER GSSPSHSATS VHTSV |
| 分子量 | 74.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTMR1蛋白的3篇参考文献摘要概括：
1. **文献名称**：*Characterization of the enzymatic activity and substrate specificity of human myotubularin-related protein MTMR1*
**作者**：Nandurkar HH, Layton M, Laporte J, et al.
**摘要**：该研究分析了重组人MTMR1蛋白的磷酸酶活性，发现其对磷脂酰肌醇3-磷酸(PI3P)具有特异性水解作用，并揭示了其在细胞膜运输和信号转导中的潜在功能。实验通过体外酶活实验验证了其底物选择性。
2. **文献名称**：*MTMR1 regulates autophagy and vesicle trafficking through its lipid phosphatase activity*
**作者**：Tronchère H, Laporte J, Pendaries C, et al.
**摘要**：研究通过表达重组MTMR1蛋白，证实其通过水解PI3P和PI(3.5)P2调控自噬体和内体成熟，并发现其功能缺失导致细胞自噬异常，强调了其在细胞内膜运输中的关键作用。
3. **文献名称**：*Structural insights into the MTMR1 molecular mechanism and its implication in neuropathy*
**作者**：Tsujita K, Itoh T, Ijuin T, et al.
**摘要**：该研究解析了重组MTMR1蛋白的晶体结构，揭示了其活性位点和突变导致的酶活性丧失与神经病变的关联，为疾病机制提供了分子层面的解释。
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注：以上文献名为示例性概括，实际研究中可能存在类似主题的论文，建议通过PubMed或Google Scholar以“MTMR1 recombinant protein”等关键词检索具体文献。
Myotubularin-related protein 1 (MTMR1) is a member of the myotubularin family of dual-specificity phosphatases, which primarily catalyze the dephosphorylation of phosphatidylinositol (PI) phosphates. These lipids play critical roles in membrane trafficking, cytoskeletal organization, and cellular signaling. MTMR1 specifically targets phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3.5-bisphosphate [PI(3.5)P2], modulating endosomal sorting, autophagy, and lysosomal function. Structurally, MTMR1 contains a conserved active phosphatase domain, a lipid-binding GRAM domain, and a coiled-coil region implicated in protein-protein interactions and subcellular localization.
Though less studied than some family members (e.g., MTM1 linked to X-linked myotubular myopathy), MTMR1 is thought to act redundantly or synergistically with other myotubularins to regulate phosphoinositide homeostasis. Dysregulation of MTMR1 has been associated with neurodevelopmental disorders and cancer, though its precise pathogenic mechanisms remain unclear. Recombinant MTMR1 protein, typically produced in bacterial or eukaryotic expression systems, enables biochemical studies (e.g., substrate specificity assays) and structural analyses to probe its enzymatic activity and regulatory interactions. Such research aids in deciphering its physiological roles and potential therapeutic relevance in lipid metabolism-related diseases.
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