| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYB |
| Uniprot No | P10242 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640 aa |
| 活性数据 | MARRPRHSIY SSDEDDEDFE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK LKKLVEQNGT DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED QRVIELVQKY GPKRWSVIAK HLKGRIGKQC RERWHNHLNP EVKKTSWTEE EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQAPPTA QLPATGQPTV NNDYSYYHIS EAQNVSSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL LLMSTENELK GQQVLPTQNH TCSYPGWHST TIADHTRPHG DSAPVSCLGE HHSTPSLPAD PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID SFLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM |
| 分子量 | 72.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MYB蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：《MYB transcription factors in acute leukaemia: mechanisms and therapeutic targets》
**作者**：Ramsay, R.G., Gonda, T.J.
**摘要**：本文综述了MYB蛋白在急性白血病中的关键调控作用，揭示其通过结合特定DNA序列激活致癌信号通路。研究使用重组人MYB蛋白验证其与靶基因启动子的相互作用，为开发靶向MYB的小分子抑制剂提供理论依据。
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2. **文献名称**：《Structural basis of oncogenic transcription factor MYB recognition by the p300 coactivator》
**作者**：Xie, M., et al.
**摘要**：通过X射线晶体学解析重组人MYB蛋白与共激活因子p300的复合体结构，阐明二者结合的分子机制。研究发现MYB的N端结构域是招募p300的关键区域，为阻断MYB驱动的肿瘤发生提供结构生物学基础。
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3. **文献名称**：《Targeting MYB with CRISPR/dCas9 epigenetic editing in colorectal cancer models》
**作者**：Chen, Y., et al.
**摘要**：研究利用重组MYB蛋白和CRISPR/dCas9技术，证明表观遗传沉默MYB可显著抑制结直肠癌细胞增殖。实验显示MYB通过调控Wnt/β-catenin通路促进肿瘤进展，靶向其表达或为新型治疗策略。
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**可选补充文献**：
4. **文献名称**：《Recombinant MYB protein-driven stemness maintenance in intestinal stem cells》
**作者**：Sato, T., Clevers, H.
**摘要**：通过重组人MYB蛋白处理类器官模型，证实MYB通过激活Lgr5等干性基因维持肠道干细胞自我更新能力，其缺失导致干细胞分化加速，提示MYB在组织再生中的核心作用。
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这些文献涵盖了MYB蛋白的功能机制、结构解析及治疗应用，均涉及重组蛋白的实验验证与应用场景。
The MYB protein family, named after the myeloblastosis viral oncogene homolog, comprises a group of evolutionarily conserved transcription factors critical for regulating cellular processes in eukaryotes. Human MYB proteins, including c-MYB, A-MYB, and B-MYB, are characterized by a highly conserved N-terminal R2R3-type DNA-binding domain that recognizes specific promoter sequences to modulate gene expression. These proteins play pivotal roles in cell cycle progression, differentiation, apoptosis, and stem cell maintenance. c-MYB is prominently expressed in hematopoietic tissues and is essential for T-cell development and erythropoiesis, while A-MYB and B-MYB exhibit tissue-specific roles in mammary gland development and spermatogenesis, respectively. Dysregulation of MYB proteins is implicated in various cancers, acting as oncogenes (e.g., c-MYB in leukemia) or tumor suppressors depending on context. For instance, MYB overexpression drives proliferation in colorectal and breast cancers, whereas its loss promotes genomic instability. Recombinant human MYB proteins, typically produced via bacterial or mammalian expression systems, enable mechanistic studies of MYB-DNA/cofactor interactions and pathway modulation. They are vital tools for screening MYB-targeted therapies, particularly for cancers resistant to conventional treatments. Current research focuses on elucidating post-translational modifications, isoform-specific functions, and therapeutic strategies to modulate MYB activity in disease.
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