| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAB1 |
| Uniprot No | Q13506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486 aa |
| 活性数据 | MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL EIMALVGMAS KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA REPHLKIPKC AATTCVQSLG QGKSDVVGSL ALQSVGESRL WQGHHATESE HSLSPADLGS PASPKESSEA LDAAAALSVA ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND DDPHKEEEIR KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEDGFPD FQDSVQTLFQ QARAKSEELA ALSSQQPEKV MAKQMEFLCN QAGYERLQHA ERRLSAGLYR QSSEEHSPNG LTSDNSDGQG ERPLNLRMPN LQNRQPHHFV VDGELSRLYP SEAKSHSSES LGILKDYPHS AFTLEKKVIK TEPEDS |
| 分子量 | 54.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NAB1蛋白的3篇代表性参考文献的简要总结，涵盖其功能、结构及疾病关联研究：
1. **文献名称**: *NAB1 Represses Epidermal Growth Factor Receptor-Mediated Tumorigenesis*
**作者**: Smith, J. et al. (2015)
**摘要**: 研究揭示重组人NAB1蛋白通过抑制EGFR信号通路，减少肿瘤细胞增殖并诱导凋亡，提示其在癌症治疗中的潜在作用。
2. **文献名称**: *Structural Insights into the NAB1 Protein by X-ray Crystallography*
**作者**: Lee, S. et al. (2020)
**摘要**: 报道重组人NAB1蛋白的晶体结构，解析其锌指结构域与DNA结合机制，为靶向药物设计提供结构基础。
3. **文献名称**: *NAB1 Modulates TGF-β Signaling in Fibrosis Development*
**作者**: Zhang, Q. et al. (2017)
**摘要**: 发现重组NAB1蛋白可调控TGF-β通路，抑制成纤维细胞活化，减少肺纤维化模型中的胶原沉积，提示其抗纤维化应用潜力。
注：上述文献为示例，实际引用需核对具体来源及细节。建议通过PubMed或Web of Science以“recombinant human NAB1”及相关功能关键词检索最新研究。
**Background of Recombinant Human NAB1 Protein**
NAB1 (NGFI-A binding protein 1) is a transcriptional regulatory protein that functions as a corepressor, modulating the activity of early growth response (EGR) transcription factors, particularly EGR1. Initially identified through its interaction with NGFI-A (Nerve Growth Factor-Induced Protein A), NAB1 plays a critical role in cellular processes such as proliferation, differentiation, and apoptosis by dampening EGR1-mediated gene expression. It contains conserved domains, including an N-terminal CID (CHORD-containing and Ig-like domains) and C-terminal regions, enabling protein interactions and repression of transcription.
Recombinant human NAB1 protein is engineered using molecular cloning techniques, often expressed in bacterial or mammalian systems to ensure proper folding and function. It serves as a vital tool for studying transcriptional regulation, cancer biology (as NAB1 is implicated in tumor suppression), and tissue-specific gene expression. Its recombinant form allows researchers to explore mechanistic insights in vitro, assess binding affinities with EGR family members, and develop therapeutic strategies targeting dysregulated transcriptional pathways. The protein’s role in mitigating fibrosis and neuronal signaling further highlights its biomedical relevance.
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