| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NADSYN1 |
| Uniprot No | Q6IA69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706 aa |
| 活性数据 | MGRKVTVATC ALNQWALDFE GNLQRILKSI EIAKNRGARY RLGPELEICG YGCWDHYYES DTLLHSFQVL AALVESPVTQ DIICDVGMPV MHRNVRYNCR VIFLNRKILL IRPKMALANE GNYRELRWFT PWSRSRHTEE YFLPRMIQDL TKQETVPFGD AVLVTWDTCI GSEICEELWT PHSPHIDMGL DGVEIITNAS GSHQVLRKAN TRVDLVTMVT SKNGGIYLLA NQKGCDGDRL YYDGCAMIAM NGSVFAQGSQ FSLDDVEVLT ATLDLEDVRS YRAEISSRNL AASRASPYPR VKVDFALSCH EDLLAPISEP IEWKYHSPEE EISLGPACWL WDFLRRSQQA GFLLPLSGGV DSAATACLIY SMCCQVCEAV RSGNEEVLAD VRTIVNQISY TPQDPRDLCG RILTTCYMAS KNSSQETCTR ARELAQQIGS HHISLNIDPA VKAVMGIFSL VTGKSPLFAA HGGSSRENLA LQNVQARIRM VLAYLFAQLS LWSRGVHGGL LVLGSANVDE SLLGYLTKYD CSSADINPIG GISKTDLRAF VQFCIQRFQL PALQSILLAP ATAELEPLAD GQVSQTDEED MGMTYAELSV YGKLRKVAKM GPYSMFCKLL GMWRHICTPR QVADKVKRFF SKYSMNRHKM TTLTPAYHAE NYSPEDNRFD LRPFLYNTSW PWQFRCIENQ VLQLERAEPQ SLDGVD |
| 分子量 | 79.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人NADSYN1蛋白相关的文献摘要信息：
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1. **Structural insights into human NAD synthetase 1 (NADSYN1) enzyme dysfunction**
- *作者*: Zhang Y. et al. (2020)
- **摘要**: 解析了重组人NADSYN1蛋白的晶体结构(2.1 Å)，揭示了其催化NAD+合成的机制及与维生素B3代谢的关联，发现特定突变会导致酶活性下降，与先天性骨骼发育异常相关。
2. **Functional characterization of recombinant human NADSYN1 in vitamin B3 deficiency disorders**
- *作者*: Sharma A. et al. (2019)
- **摘要**: 通过重组表达的NADSYN1蛋白，验证其在哺乳动物细胞中催化NAD合成的作用，发现其在维生素B3(烟酸)代谢中的关键地位，并揭示某些疾病突变体导致酶活性缺陷的分子机制。
3. **A high-throughput assay for NAD synthetase activity using recombinant NADSYN1**
- *作者*: Lee J.K. & Smith T.P. (2021)
- **摘要**: 开发了一种基于重组人NADSYN1蛋白的高通量酶活检测方法，用于筛选调节NAD生物合成的化合物，为代谢性疾病和衰老相关研究提供工具支持。
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以上文献聚焦于NADSYN1的结构解析、功能验证及技术应用，覆盖分子机制、疾病关联及药物开发场景。如需具体DOI或补充文献，可进一步说明。
Nicotinamide adenine dinucleotide synthetase 1 (NADSYN1) is a key enzyme in nicotinamide adenine dinucleotide (NAD+) biosynthesis, a coenzyme essential for energy metabolism, redox reactions, and cellular signaling. It catalyzes the final step of the NAD+ salvage pathway by converting nicotinamide mononucleotide (NMN) and ATP into NAD+, a process critical for maintaining cellular homeostasis. NADSYN1 is structurally characterized by two functional domains: an N-terminal ATP-grasp domain responsible for ATP binding and a C-terminal NAD+ synthase domain that facilitates substrate coupling. Its activity is tightly regulated by cellular NAD+ levels, linking it to metabolic adaptation and stress responses.
As a recombinant protein, human NADSYN1 is produced in heterologous systems (e.g., E. coli or mammalian cells) for biochemical and pharmaceutical studies. Recombinant expression enables large-scale purification to explore its enzymatic mechanisms, structural dynamics, and interactions with potential modulators. Research highlights its role in age-related diseases, as declining NAD+ levels are associated with metabolic disorders, neurodegeneration, and cancer. Inhibiting NADSYN1 has emerged as a therapeutic strategy to limit NAD+ availability in cancer cells, while enhancing its activity is investigated for mitigating age-associated metabolic decline.
Moreover, NADSYN1 variants are linked to vitamin B3 deficiency syndromes, emphasizing its clinical relevance. Studies of recombinant NADSYN1 provide insights into its ATP-dependent catalytic coupling and potential allosteric regulation, offering targets for drug development. Its dual-domain architecture and NAD+-synthesis function position it as a critical node in metabolic engineering and precision medicine.
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