| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NANOS1 |
| Uniprot No | Q8WY41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292 aa |
| 活性数据 | MEAFPWAPRS PRRGRAPPPM ALVPSARYVS APGPAHPQPF SSWNDYLGLA TLITKAVDGE PRFGCARGGN GGGGSPPSSS SSSCCSPHTG AGPGALGPAL GPPDYDEDDD DDSDEPGSRG RYLGSALELR ALELCAGPAE AGLLEERFAE LSPFAGRAAA VLLGCAPAAA AAATTTSEAT PREERAPAWA AEPRLHAASG AAAARLLKPE LQVCVFCRNN KEAMALYTTH ILKGPDGRVL CPVLRRYTCP LCGASGDNAH TIKYCPLSKV PPPPARPPPR SARDGPPGKK LR |
| 分子量 | 30.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NANOS1蛋白的3篇文献参考(文献为示例性整理，具体内容可能需要根据实际发表论文调整)：
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1. **标题**: "Expression and Functional Characterization of Recombinant Human NANOS1 in Germ Cell Development"
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统成功表达重组人NANOS1蛋白，并验证其在体外结合特定RNA序列的能力，揭示了其在生殖细胞分化中的潜在调控作用。
2. **标题**: "NANOS1 as a Cancer Biomarker: Production and Validation of Recombinant Protein in HEK293 Cells"
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物表达系统(HEK293)制备高纯度重组NANOS1蛋白，通过免疫组化证实其在多种肿瘤组织中高表达，提示其作为癌症诊断标志物的可能性。
3. **标题**: "Structural Insights into RNA Recognition by Human NANOS1 Protein"
**作者**: Tanaka K, et al.
**摘要**: 解析重组NANOS1蛋白的晶体结构，鉴定其锌指结构域对靶标RNA的结合机制，为理解胚胎发育中NANOS1的功能提供分子基础。
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**备注**：上述文献信息为示例，实际文献需通过PubMed、Web of Science或Google Scholar检索关键词“recombinant human NANOS1”获取。如需具体文献，可提供更详细的研究方向(如表达体系、功能领域等)。
NANOS1 is a member of the evolutionarily conserved NANOS protein family, which plays critical roles in germ cell development, stem cell maintenance, and embryogenesis across species. In humans, NANOS1 encodes a RNA-binding protein characterized by a conserved C-terminal zinc-finger domain that mediates interactions with target mRNAs and protein partners like Pumilio family proteins. It is predominantly expressed in germline cells and certain somatic tissues, where it regulates gene expression post-transcriptionally by influencing mRNA stability, translation, or localization.
Recombinant human NANOS1 protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Purified NANOS1 facilitates research into its roles in gametogenesis, neural development, and cancer progression, as dysregulation is linked to infertility, neurodevelopmental disorders, and malignancies. For instance, NANOS1 overexpression has been implicated in tumor invasion and metastasis in cancers like hepatocellular carcinoma, while its deficiency may disrupt spermatogenesis. Despite progress, challenges remain in mapping its precise RNA targets, dynamic regulatory networks, and context-dependent dual roles (oncogenic vs. tumor-suppressive). Further studies on recombinant NANOS1 could advance its potential as a therapeutic target or biomarker for reproductive or oncological conditions.
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