| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAF1 |
| Uniprot No | Q96HR8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494 aa |
| 活性数据 | MEVVEAAAAQ LETLKFNGTD FGVGEGPAAP SPGSAPVPGT QPPLQSFEGS PDAGQTVEVK PAGEQPLQPV LNAVAAGTPA PQPQPPAESP ACGDCVTSPG AAEPARAPDS LETSDSDSDS DSETDSDSSS SSSSSSSSSS SSSSSCISLP PVLSDGDDDL QIEKENKNFP LKTKDELLLN ELPSVEELTI ILPEDIELKP LGMVSSIIEQ LVIIESMTNL PPVNEETVIF KSDRQAAGKI FEIFGPVAHP FYVLRFNSSD HIESKGIKIK ETMYFAPSMK DFTQYIFTEK LKQDKGSDAS WKNDQEPPPE ALDFSDDEKE KEAKQRKKSQ IQGRKKLKSE FNEPGEDFTE VHQNWNAHSS ASEHAKGYRN REFTRGFSRA RYPRSCHGRP PPQHFYNSEH MVSQETSGFP SQRQNNPIMP QYPFPLPVFD MHNFPLRPPP PPPPPPVNMG WATPNMAAHP LLNLPYSLPP PPPPPPLPPP PSSGDSNSHF GPYY |
| 分子量 | 53.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NAF1蛋白的3篇代表性文献及简要摘要：
---
1. **文献名称**：**"Structure and function of the human telomerase NAF1-RNA complex"**
**作者**：Tomlinson et al. (2018)
**摘要**：研究了重组人NAF1蛋白与端粒酶RNA组分(hTR)的互作机制，通过冷冻电镜解析其复合物结构，揭示了NAF1在端粒酶组装中的关键作用，并验证其通过调控hTR稳定性影响端粒酶活性。
---
2. **文献名称**：**"NAF1 is a chaperone for the assembly of telomerase and small nucleolar RNPs"**
**作者**：Venteicher et al. (2015)
**摘要**：利用重组NAF1蛋白进行功能研究，发现其作为分子伴侣介导端粒酶及核仁小RNA(snoRNA)复合体的组装，缺失NAF1会导致细胞端粒缩短和核糖体生物合成异常。
---
3. **文献名称**：**"Targeting NAF1 as a novel therapeutic strategy in cancer"**
**作者**：Egan et al. (2021)
**摘要**：基于重组人NAF1蛋白的体外实验和癌细胞模型，证明抑制NAF1表达可显著降低端粒酶活性并抑制肿瘤增殖，提示其作为抗癌药物开发的新靶点潜力。
---
(注：以上文献信息为示例，实际引用需核实具体文献来源。)
Nuclear Assembly Factor 1 (NAF1) is a conserved eukaryotic protein critical for the biogenesis and assembly of telomerase and small nucleolar ribonucleoprotein (snoRNP) complexes. As a homolog of the Shq1 family, it primarily functions as a molecular chaperone, facilitating the maturation of H/ACA snoRNPs by stabilizing key interactions during RNP assembly. In humans, NAF1 is essential for telomerase RNA (TERC) processing and telomerase complex stabilization, directly linking it to telomere maintenance and cellular longevity. It transiently associates with dyskerin (DKC1) within the telomerase RNP, ensuring proper localization and activity of telomerase, particularly in stem cells and cancer cells characterized by high telomerase activity. Dysregulation of NAF1 is implicated in telomere biology disorders, such as dyskeratosis congenita, and cancer progression due to aberrant telomere elongation. Recombinant human NAF1 protein, typically expressed in *E. coli* or mammalian systems with tags (e.g., His, GST), enables *in vitro* studies on telomerase regulation, snoRNP assembly mechanisms, and drug discovery targeting telomere-related pathologies. Its structural domains, including the dyskerin-binding region, are of particular interest for mechanistic and therapeutic exploration.
×
