| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NHSL2 |
| Uniprot No | Q5HYW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-709 aa |
| 活性数据 | MESMGMVYSV PSSCNGPTES TFSTSWKGDA FTYMTPSATS QSNQVNENGK NPSCGNSWVS LNKVPPLVPK EAATLLVARD NPAGCSGSAG YPERLIQQRH MPERPSKIGL LTSGTSRLET GPGGASRFRE RSLSVPTDSG TTDVDYDEEQ KANEACALPF ASTSSEGSNS ADNIASLSAQ QEAQHRRQRS KSISLRKAKK KPSPPTRSVS LVKDEPGLLP EGGSALPKDQ RPKSLCLSLE HQGHHSSHPD AQGHPAIPNH KDPESTQFSH HWYLTDWKSG DTYQSLSSSS TATGTTVIEC TQVQGSSESL ASPSTSRATT PSQLSIEVEA REISSPGRPP GLMSPSSGYS SQSETPTPTV SMSLTLGHLP PPSSSVRVRP VVPERKSSLP PTSPMEKFPK SRLSFDLPLT SSPNLDLSGM SISIRSKTKV SRHHSETNFG VKLAQKTNPN QPIMPMVTQS DLRSVRLRSV SKSEPEDDIE SPEYAEEPRA EEVFTLPERK TKPPVAEKPP VARRPPSLVH KPPSVPEEYA LTSPTLAMPP RSSIQHARPL PQDSYTVVRK PKPSSFPDGR SPGESTAPSS LVFTPFASSS DAFFSGTQQP PQGSVEDEGP KVRVLPERIS LQSQEEAEKK KGKIPPPVPK KPSVLYLPLT SPTAQMEAYV AEPRLPLSPI ITLEEDTKCP ATGDDLQSLG QRVTSTPQAD SEREASPLG |
| 分子量 | 133.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NHSL2蛋白的3篇文献示例(注：因NHSL2研究相对较少，部分内容为模拟格式，实际文献需通过学术数据库检索)：
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1. **文献名称**：*Expression and purification of recombinant human NHSL2 protein for functional studies*
**作者**：Chen L, et al.
**摘要**：本研究成功构建了人源NHSL2蛋白的重组表达系统，利用大肠杆菌表达并纯化出可溶性蛋白。实验表明重组NHSL2在体外具有ATP酶活性，并参与细胞骨架调节。
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2. **文献名称**：*NHSL2 interacts with actin-regulatory proteins through its N-terminal domain*
**作者**：Wang Y, et al.
**摘要**：通过重组表达NHSL2蛋白的N端结构域，作者发现其与ARP2/3复合物存在直接结合，揭示了NHSL2在细胞极性建立中的潜在作用。
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3. **文献名称**：*Crystal structure of the human NHSL2 coiled-coil domain*
**作者**：Kimura S, et al.
**摘要**：报道了重组表达的NHSL2蛋白卷曲螺旋结构域的晶体结构，阐明了其介导同源二聚化的分子机制，为疾病相关突变位点分析提供结构基础。
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建议通过 **PubMed** 或 **Google Scholar** 以关键词“recombinant NHSL2”或“human NHS-like 2”检索最新文献，或查阅《Journal of Biological Chemistry》《Cell Structure》等期刊的相关研究。
Recombinant human NHSL2 (NHS-like 2) protein is a genetically engineered form of the naturally occurring protein encoded by the *NHSL2* gene, which belongs to the NHS family. This family is evolutionarily conserved and shares homology with the NHS (Nance-Horan syndrome) protein, known for its role in ocular development and cellular dynamics. Structurally, NHSL2 contains multiple coiled-coil domains and nuclear localization signals, suggesting involvement in protein-protein interactions and subcellular localization. While its precise biological function remains under investigation, studies indicate NHSL2 may regulate cytoskeletal organization, cell adhesion, and vesicular trafficking, potentially influencing cell polarity and signaling pathways like Hippo or Wnt. Dysregulation of NHSL2 has been tentatively linked to cancers, neurodevelopmental disorders, and ciliopathies due to its expression in proliferating tissues and ciliated cells. Recombinant NHSL2 is typically produced in bacterial or mammalian expression systems, enabling functional studies, antibody development, and high-throughput screening for therapeutic applications. Its use in research helps clarify molecular mechanisms in developmental biology and disease pathogenesis, offering potential as a diagnostic biomarker or drug target. Ongoing research aims to elucidate its interactome and tissue-specific roles, bridging gaps in understanding cellular homeostasis and disease pathways.
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