| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NGEF |
| Uniprot No | Q8N5V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-710 aa |
| 活性数据 | METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR MPPCRTAMQT DPGAQEMSES SSTPGNGATP EEWPALADSP TTLTEALRMI HPIPADSWRN LIEQIGLLYQ EYRDKSTLQE IETRRQQDAE IEDNTNGSPA SEDTPEEEEE EEEEEEPASP PERKTLPQIC LLSNPHSRFN LWQDLPEIRS SGVLEILQPE EIKLQEAMFE LVTSEASYYK SLNLLVSHFM ENERIRKILH PSEAHILFSN VLDVLAVSER FLLELEHRME ENIVISDVCD IVYRYAADHF SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC RGLPFSSFLI LPFQRITRLK LLVQNILKRV EERSERECTA LDAHKELEMV VKACNEGVRK MSRTEQMISI QKKMEFKIKS VPIISHSRWL LKQGELQQMS GPKTSRTLRT KKLFHEIYLF LFNDLLVICR QIPGDKYQVF DSAPRGLLRV EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILDKTDDGWI FGERLHDQER GWFPSSMTEE ILNPKIRSQN LKECFRVHKM DDPQRSQNKD RRKLGSRNRQ |
| 分子量 | 82.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NGEF蛋白的3篇参考文献示例(注：文献信息为模拟示例，非真实存在)：
1. **"Expression and functional characterization of recombinant human NGEF in neuronal development"**
- **作者**: Li X, Wang Y, et al.
- **摘要**: 研究利用大肠杆菌表达系统成功制备重组人NGEF蛋白，验证其通过激活RhoA信号通路促进神经突触生长和神经元分化，揭示了NGEF在中枢神经系统发育中的作用。
2. **"NGEF interacts with amyloid precursor protein and regulates Alzheimer's disease pathology"**
- **作者**: Zhang R, Chen H, et al.
- **摘要**: 通过哺乳动物细胞表达重组人NGEF蛋白，发现其与APP(淀粉样前体蛋白)的相互作用可抑制β-分泌酶活性，减少Aβ生成，为阿尔茨海默病的治疗提供了潜在靶点。
3. **"Recombinant NGEF promotes glioma cell invasion via activation of Rho/ROCK pathway"**
- **作者**: Gupta S, Patel K, et al.
- **摘要**: 在胶质瘤细胞中过表达重组人NGEF蛋白，证实其通过激活Rho/ROCK通路增强肿瘤细胞迁移和侵袭能力，提示NGEF可能成为胶质瘤治疗的分子靶标。
(如需真实文献，建议通过PubMed或Google Scholar搜索“recombinant NGEF”、“Arhgef2”等关键词获取。)
Recombinant human Neuronal Guanine nucleotide Exchange Factor (NGEF) protein is a genetically engineered protein derived from the human NGEF gene, which encodes a critical regulator of Rho GTPase signaling pathways. NGEF belongs to the Dbl family of guanine nucleotide exchange factors (GEFs), characterized by conserved DH (Dbl homology) and PH (Pleckstrin homology) domains. It primarily activates Rho GTPases, such as RhoA and Cdc42. by facilitating the exchange of GDP for GTP, thereby modulating cytoskeletal dynamics, cell migration, and neuronal development.
NGEF is prominently expressed in the central nervous system, where it influences axon guidance, dendritic arborization, and synaptic plasticity. Dysregulation of NGEF has been implicated in neurological disorders, including Alzheimer’s disease, schizophrenia, and intellectual disabilities, highlighting its role in maintaining neural circuit integrity. Recombinant NGEF protein is produced via bacterial or mammalian expression systems, enabling studies on its structural properties, interaction networks, and mechanistic contributions to cellular signaling. Its application spans in vitro assays, drug screening, and disease modeling, offering insights into therapeutic strategies targeting Rho GTPase-related pathologies. Ongoing research aims to elucidate its tissue-specific functions and potential as a biomarker or therapeutic target in neurodegenerative and psychiatric conditions.
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