| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRNR |
| Uniprot No | Q86YZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于HRNR(Hornerin)重组蛋白的模拟参考文献示例(真实文献需通过学术数据库查询)：
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1. **文献名称**：*"Recombinant Hornerin Protein Enhances Epidermal Barrier Function in Atopic Dermatitis Models"*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组HRNR蛋白，发现其可促进角质形成细胞分化，并通过调控紧密连接蛋白改善特应性皮炎小鼠模型的皮肤屏障功能。
2. **文献名称**：*"Antimicrobial Properties of HRNR-Derived Peptides: A Structural and Functional Analysis"*
**作者**：Chen L, et al.
**摘要**：通过重组HRNR蛋白的酶解获得多肽片段，实验证实其对金黄色葡萄球菌和白色念珠菌具有显著抑制活性，揭示了HRNR在先天免疫中的潜在抗菌机制。
3. **文献名称**：*"HRNR Expression in Psoriasis: Recombinant Protein-Based Biomarker Screening"*
**作者**：Kumar R, et al.
**摘要**：利用重组HRNR蛋白开发ELISA检测方法，发现银屑病患者血清中HRNR水平异常升高，提示其可作为炎症性皮肤病的生物标志物。
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**注意**：以上文献为模拟示例，实际研究需参考PubMed、Web of Science等平台(关键词：Hornerin、HRNR recombinant protein、skin barrier、antimicrobial peptide)。建议结合具体研究方向补充检索。
**Background of HRNR Recombinant Protein**
HRNR (Hornerin) is a member of the S100-fused-type protein (SFTP) family, primarily expressed in the epidermis and associated with skin barrier formation and innate immune responses. Structurally, it contains EF-hand calcium-binding domains and repetitive glycine-rich motifs, resembling other epidermal proteins like filaggrin and involucrin. These features enable HRNR to interact with keratin intermediate filaments, contributing to cornified envelope assembly and maintaining epidermal integrity.
Functionally, HRNR exhibits antimicrobial properties, particularly against *Staphylococcus aureus*, and modulates inflammatory pathways by interacting with immune receptors (e.g., TLR2). It also influences keratinocyte differentiation and wound healing. Dysregulation of HRNR is linked to dermatological disorders such as atopic dermatitis (AD) and psoriasis, where reduced expression or genetic mutations correlate with impaired barrier function and chronic inflammation.
Recombinant HRNR protein is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, followed by purification via affinity chromatography. Its applications span *in vitro* studies to dissect molecular mechanisms in skin biology, drug screening for inflammatory diseases, and development of topical therapeutics or diagnostic biomarkers. Additionally, engineered HRNR variants are explored for enhancing stability or targeting specific pathways.
Overall, HRNR recombinant protein serves as a critical tool for advancing dermatological research and therapeutic innovation.
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