| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOC2L |
| Uniprot No | Q9Y3T9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749 aa |
| 活性数据 | MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSAAFNALVTFCIRDLIGCLQKLLFGKVAKDSSRMLQPSSSPLWGKLRVDIKAYLGSAIQLVSCLSETTVLAAVLRHISVLVPCFLTFPKQCRMLLKRMVVVWSTGEESLRVLAFLVLSRVCRHKKDTFLGPVLKQMYITYVRNCKFTSPGALPFISFMQWTLTELLALEPGVAYQHAFLYIRQLAIHLRNAMTTRKKETYQSVYNWQYVHCLFLWCRVLSTAGPSEALQPLVYPLAQVIIGCIKLIPTARFYPLRMHCIRALTLLSGSSGAFIPVLPFILEMFQQVDFNRKPGRMSSKPINFSVILKLSNVNLQEKAYRDGLVEQLYDLTLEYLHSQAHCIGFPELVLPVVLQLKSFLRECKVANYCRQVQQLLGKVQENSAYICSRRQRVSFGVSEQQAVEAWEKLTREEGTPLTLYYSHWRKLRDREIQLEISGKERLEDLNFPEIKRRKMADRKDEDRKQFKDLFDLNSSEEDDTEGFSERGILRPLSTRHGVEDDEEDEEEGEEDSSNSEDGDPDAEAGLAPGELQQLAQGPEDELEDLQLSEDD |
| 分子量 | 111.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOC2L蛋白的模拟参考文献示例(具体文献需通过学术数据库验证)：
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1. **文献名称**：*NOC2L regulates leukemic cell proliferation via histone modification pathways*
**作者**：Wang, X. et al. (2020)
**摘要**：研究报道NOC2L在急性髓系白血病中异常高表达，重组人NOC2L蛋白通过调控组蛋白H3K4甲基化修饰抑制癌细胞增殖，揭示其在表观遗传调控中的潜在治疗价值。
2. **文献名称**：*Structural insights into the interaction of NOC2L with autophagy-related protein LC3*
**作者**：Saito, T. et al. (2018)
**摘要**：通过解析重组NOC2L蛋白的晶体结构，发现其C端结构域可直接结合LC3蛋白，提出NOC2L在细胞自噬应激反应中的分子机制。
3. **文献名称**：*NOC2L acts as a tumor suppressor in hepatocellular carcinoma through modulating p53 stability*
**作者**：Chen, L. et al. (2019)
**摘要**：利用重组NOC2L蛋白进行功能研究，证实其通过泛素化途径稳定p53蛋白，抑制肝癌细胞增殖并促进凋亡，提示其作为肿瘤标志物的潜力。
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**备注**：以上为示例性内容，实际文献需查询PubMed、Web of Science等平台。建议使用关键词“recombinant NOC2L protein”或结合具体研究方向(如癌症、自噬、结构生物学)进一步筛选。
**Background of Recombinant Human NOC2L Protein**
NOC2L (Nucleolar Complex Protein 2 Homolog) is a conserved eukaryotic protein implicated in diverse cellular processes, including ribosome biogenesis, cell proliferation, and DNA damage response. It shares homology with yeast Noc2p, a factor involved in ribosomal RNA maturation and nucleolar-cytoplasmic transport. In humans, NOC2L is ubiquitously expressed and localizes predominantly to the nucleolus, though it may shuttle between nuclear and cytoplasmic compartments depending on cellular context.
Studies suggest NOC2L interacts with key proteins like RPL23 (a ribosomal component) and UVRAG, linking it to ribosome assembly, autophagy, and genomic stability. Its role in cell cycle regulation, particularly at the G1/S transition, highlights its potential relevance in cancer biology. Dysregulation of NOC2L has been observed in certain malignancies and neurological disorders, though its precise mechanistic contributions remain under investigation.
Recombinant NOC2L protein, produced via expression systems like *E. coli* or mammalian cells, enables functional and structural studies. Researchers utilize it to explore its binding partners, post-translational modifications, and enzymatic activities. Additionally, recombinant NOC2L aids in deciphering its interplay with pathways such as mTOR signaling and stress responses. As a tool, it contributes to drug discovery efforts targeting diseases associated with ribosomal dysfunction or uncontrolled proliferation. Current research continues to unravel its dual roles in homeostasis and pathology, positioning NOC2L as a molecule of interest in both basic and applied biomedical sciences.
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