| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMUR2 |
| Uniprot No | Q9GZQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415 aa |
| 活性数据 | MSGMEKLQNASWIYQQKLEDPFQKHLNSTEEYLAFLCGPRRSHFFLPVSVVYVPIFVVGVIGNVLVCLVILQHQAMKTPTNYYLFSLAVSDLLVLLLGMPLEVYEMWRNYPFLFGPVGCYFKTALFETVCFASILSITTVSVERYVAILHPFRAKLQSTRRRALRILGIVWGFSVLFSLPNTSIHGIKFHYFPNGSLVPGSATFTVIKPMWIYNFIIQVTSFLFYLLPMTVISVLYYLMALRLKKDKSLEADEGNANIQRPCRKSVNKMLFVLVLVFAICWAPFHIDRLFFSFVEEWSESLAAVFNLVHVVSGVFFYLSSAVNPIIYNLLSRRFQAAFQNVISSFHKQWHSQHDPQLPPAQRNIFLTECHFVELTEDIGPQFPCQSSMHNSHLPTALSSEQMSRTNYQSFHFNKT |
| 分子量 | 71.39 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的重组人NMUR2蛋白相关参考文献摘要，供参考：
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1. **文献名称**：*"Cloning and Functional Characterization of the Human Neuromedin U Receptor 2 (NMUR2)"*
**作者**：Mitchell, R. et al.
**摘要**：该研究首次成功克隆人源NMUR2受体基因，并在哺乳动物细胞中表达重组蛋白。实验表明NMUR2通过Gq蛋白偶联通路激活细胞内钙信号，并在下丘脑和脊髓中高度表达，提示其在调节摄食和疼痛感知中的作用。
2. **文献名称**：*"Recombinant NMUR2 Expression and Ligand Binding Specificity in Neuronal Tissues"*
**作者**：Brighton, P. J. et al.
**摘要**：通过构建重组NMUR2蛋白，揭示了其对内源性神经肽Neuromedin U(NMU)的高亲和力结合特性，并证实其在自主神经系统中的广泛分布，为研究NMUR2在应激反应中的功能提供了依据。
3. **文献名称**：*"Cryo-EM Structure of NMUR2 in Complex with Agonist Reveals Activation Mechanism"*
**作者**：Xu, X. et al.
**摘要**：利用冷冻电镜技术解析了重组NMUR2蛋白与激动剂NMU的结合构象，阐明受体跨膜结构域的动态变化及其与下游信号蛋白的相互作用机制，为靶向药物设计提供结构基础。
4. **文献名称**：*"NMUR2 Knockout Mice and Recombinant Receptor Studies Identify Metabolic Regulation Pathways"*
**作者**：Hedrick, J. A. et al.
**摘要**：结合基因敲除模型和体外重组NMUR2功能实验，发现NMUR2通过调节瘦素敏感性影响能量代谢，提示其作为肥胖治疗靶点的潜力。
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**注**：以上文献为示例性内容，实际研究中建议通过学术数据库(如PubMed、Web of Science)检索具体论文。
Neuromedin U receptor 2 (NMUR2) is a G-protein-coupled receptor (GPCR) that binds to neuromedin U (NmU), a neuropeptide widely expressed in the central nervous system and peripheral tissues. NMUR2. encoded by the *NMUR2* gene, is primarily localized in the brain, particularly in regions like the hypothalamus, brainstem, and spinal cord, suggesting its involvement in regulating energy homeostasis, stress responses, and nociception. Unlike its homolog NMUR1. which is predominantly found in peripheral tissues, NMUR2 plays a more critical role in central neurotransmission. The receptor activates intracellular signaling pathways, including Gαq-mediated phospholipase C (PLC) activation, leading to calcium mobilization and downstream effects on neuronal excitability and neurotransmitter release.
Recombinant human NMUR2 protein is engineered for research to study receptor-ligand interactions, signaling mechanisms, and potential therapeutic applications. Its production typically involves heterologous expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional activity. Studies using recombinant NMUR2 have highlighted its relevance in appetite regulation, metabolic disorders, and inflammatory pain. Dysregulation of NMUR2 signaling has been implicated in obesity, anxiety, and irritable bowel syndrome, making it a target for drug development. Current research focuses on identifying selective agonists/antagonists and elucidating its structural biology to design novel therapeutics for metabolic and neurological conditions.
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