| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NNAT |
| Uniprot No | Q16517 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-81 aa |
| 活性数据 | MAAVAAASAE LLIIGWYIFR VLLQVFLECC IYWVGFAFRN PPGTQPIARS EVFRYSLQKL AYTVSRTGRQ VLGERRQRAP N |
| 分子量 | 9.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NNAT蛋白的参考文献范例(注：以下内容为学术示例格式，非真实文献)：
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1. **文献名称**: "Expression and Purification of Recombinant Human Neuronatin (NNAT) in E. coli"
**作者**: Zhang L, Wang Y, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达重组人NNAT蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并利用Western Blot验证其特异性，为后续功能研究奠定基础。
2. **文献名称**: "Functional Analysis of NNAT in Calcium Homeostasis Regulation"
**作者**: Smith JA, Tanaka R, et al.
**摘要**: 通过体外细胞模型证实重组人NNAT蛋白通过调节内质网钙离子通道影响细胞内钙稳态，提示其在神经发育及疾病中的潜在作用。
3. **文献名称**: "Structural Characterization of Human Neuronatin by X-ray Crystallography"
**作者**: Gupta S, Müller P, et al.
**摘要**: 首次解析了重组人NNAT蛋白的晶体结构，揭示其疏水结构域与膜结合能力的关系，为探究其生理功能提供分子机制依据。
4. **文献名称**: "NNAT as a Potential Biomarker in Neurodegenerative Diseases"
**作者**: Chen H, Li X, et al.
**摘要**: 研究利用重组NNAT蛋白进行血清学检测，发现其在阿尔茨海默病患者中表达异常，提示其作为神经退行性疾病生物标志物的可能性。
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(提示：以上文献为模拟示例，实际写作需引用真实发表的论文资源。)
Neuronatin (NNAT) is a small, highly conserved protein encoded by the *NNAT* gene, located on human chromosome 20q11.2. First identified for its role in neural and endocrine system development, NNAT is predominantly expressed in embryonic and neonatal tissues, particularly the brain, pancreas, and adipose tissue. It exists as two splice variants, α and β, differing by a 21-amino acid segment. Structurally, NNAT is a transmembrane protein hypothesized to regulate ion channel activity or act as a molecular chaperone during cellular stress.
Functionally, NNAT is implicated in neuronal differentiation, cell proliferation, and apoptosis. It plays a critical role in maintaining calcium homeostasis, influencing both neurodevelopment and metabolic processes. Dysregulation of NNAT has been linked to developmental disorders, obesity, and cancer. For instance, its overexpression is observed in gliomas and neuroblastomas, while epigenetic silencing (via promoter methylation) is associated with metabolic syndrome and pancreatic dysfunction.
Recombinant human NNAT protein is typically produced using *E. coli* or mammalian expression systems, enabling studies on its biochemical properties and interactions. Its applications span basic research—elucidating neural development pathways—to translational studies targeting NNAT-related disorders. However, its precise molecular mechanisms remain under investigation, highlighting its potential as a therapeutic target or biomarker in neurological and metabolic diseases.
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