关键信息
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基因名
Cfi
- 应用
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别名
Cfi；IF；Complement factor I
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05156
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表达区间
239-316aa
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氨基酸序列
KACDGINDCGDQSDELCCKACQGKGFHCKSGVCIPSQYQCNGEVDCITGEDEVGCAGFASVTQEETEILTADMDAERR
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分子量
43.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Cfi, or Cyclophilin A, is a well-studied protein known for its role as a peptidyl-prolyl isomerase and its involvement in various cellular functions, including protein folding, trafficking, and regulation of immune responses. Research on Cfi has gained momentum due to its implications in several diseases, particularly in HIV, where it aids viral replication by interacting with viral proteins. Furthermore, Cfi has been implicated in cancer progression, neurodegenerative diseases, and inflammation, highlighting its potential as a therapeutic target. Advances in molecular biology and structural biology techniques have paved the way for the detailed study of Cfi and its interactions within cellular pathways. The re-engineering of Cfi to create recombinant proteins has enabled scientists to explore its structural dynamics and functional mechanisms more thoroughly. By producing Cfi in heterologous systems, researchers can generate large quantities of the protein for functional assays and high-resolution structural analyses, facilitating a deeper understanding of its role in disease pathology and paving the way for the development of small-molecule inhibitors or other therapeutics aimed at modulating its activity. Investigating the various isoforms and post-translational modifications of Cfi could also shed light on its multifaceted roles within the cell. Overall, the study of Cfi as a recombinant protein represents a significant avenue in biomedical research, promising novel insights into its functions and potential therapeutic applications.
