| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPRS1 |
| Uniprot No | Q99720 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223 aa |
| 活性数据 | MQWAVGRRWAWAALLLAVAAVLTQVVWLWLGTQSFVFQREEIAQLARQYAGLDHELAFSRLIVELRRLHPGHVLPDEELQWVFVNAGGWMGAMCLLHASLSEYVLLFGTALGSRGHSGRYWAEISDTIISGTFHQWREGTTKSEVFYPGETVVHGPGEATAVEWGPNTWMVEYGRGVIPSTLAFALADTVFSTQDFLTLFYTLRSYARGLRLELTTYLFGQDP |
| 分子量 | 51.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OPRS1蛋白的假设性参考文献示例(注：OPRS1并非广泛研究的蛋白名称，以下内容为模拟示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: "Cloning and Functional Analysis of Recombinant Human OPRS1 Protein in Cellular Antioxidant Pathways"
**作者**: Smith A, et al.
**摘要**: 本研究克隆了人源OPRS1基因，通过大肠杆菌系统表达并纯化了重组蛋白。功能实验表明，OPRS1通过调节硫氧还蛋白通路减轻氧化应激，可能成为抗氧化治疗的潜在靶点。
2. **文献名称**: "Structural Characterization of OPRS1 and Its Role in Selenium Metabolism"
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组OPRS1的3D结构，揭示了其依赖硒半胱氨酸的催化机制，为理解其在硒代谢和甲状腺功能中的作用提供了结构基础。
3. **文献名称**: "High-Yield Production of Recombinant OPRS1 Using a Mammalian Expression System"
**作者**: Wang Q, et al.
**摘要**: 开发了一种基于HEK293细胞的重组OPRS1蛋白高效表达及纯化方法，获得的蛋白具有正确翻译后修饰，适用于体外酶活性和药物筛选研究。
4. **文献名称**: "OPRS1 Overexpression Correlates with Poor Prognosis in Colorectal Cancer"
**作者**: Garcia R, et al.
**摘要**: 临床样本分析表明，OPRS1在结直肠癌中高表达，重组蛋白实验显示其通过激活MAPK通路促进肿瘤细胞增殖，提示其作为生物标志物的潜力。
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**注意**：OPRS1可能为示例蛋白名称，若实际研究中存在拼写差异(如SEPHS1、OPA1等)，建议通过数据库(如PubMed、Google Scholar)结合具体研究方向检索确认。
**Recombinant human OPRS1 protein** is a genetically engineered form of the opioid receptor-like 1 (OPRL1 or OPRS1), a G protein-coupled receptor (GPCR) encoded by the *OPRL1* gene in humans. Originally identified as a homolog of opioid receptors, OPRS1 binds the endogenous neuropeptide nociceptin/orphanin FQ (N/OFQ), playing a critical role in modulating pain perception, stress responses, anxiety, and reward pathways. Unlike classical opioid receptors (e.g., μ, δ, κ), OPRS1 activation typically inhibits neurotransmitter release via Gi/o protein signaling, influencing neural circuits involved in emotional and physiological regulation.
Recombinant OPRS1 is produced in heterologous expression systems (e.g., HEK293. insect cells) to study receptor-ligand interactions, signaling mechanisms, and drug discovery. Its purified form enables structural studies (e.g., cryo-EM, X-ray crystallography) to elucidate binding sites and conformational changes. Research highlights its therapeutic potential in pain management, addiction, and mood disorders, as both agonists and antagonists are explored to fine-tune its activity. Dysregulation of OPRS1 is linked to chronic pain conditions, depression, and neurodegenerative diseases, making it a biomarker and target for precision medicine. Studies using recombinant OPRS1 have advanced understanding of nociceptin system complexity and cross-talk with opioid pathways, offering alternatives to traditional opioids with reduced side-effect profiles.
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